RT Journal Article SR Electronic(1) A1 Zhang, Yun A1 Ma, Ying-Fei A1 Qi, Su-Wei A1 Meng, Bo A1 Chaudhry, Muhammad Tausif A1 Liu, Si-Qi A1 Liu, Shuang-JiangYR 2007 T1 Responses to arsenate stress by Comamonas sp. strain CNB-1 at genetic and proteomic levels JF Microbiology, VO 153 IS 11 SP 3713 OP 3721 DO https://doi.org/10.1099/mic.0.2007/011403-0 PB Microbiology Society, SN 1465-2080, AB Comamonas sp. strain CNB-1, a chloronitrobenzene-degrading bacterium, was demonstrated to possess higher arsenate tolerance as compared with the mutant strain CNB-2. pCNB1, a plasmid harboured by CNB-1 but not CNB-2, contained the genetic cluster ars(RPBC)Com , which putatively encodes arsenate-resistance regulator, family II arsenate reductase, arsenite efflux pump and family I arsenate reductase, respectively, in Comamonas strain CNB-1. The arsC-negative Escherichia coli could gain arsenate resistance by transformation with arsPCom or arsCCom , indicating that these two genes might express functional forms of arsenate reductases. Intriguingly, when CNB-1 cells were exposed to arsenate, the transcription of arsPCom and arsCCom was measurable by RT-PCR, but only ArsP Com was detectable at protein level. To explore the proteins responding to arsenate stress, CNB-1 cells were cultured with and without arsenate and differential proteomics was carried out by two-dimensional PAGE (2-DE) and MALDI-TOF MS. A total of 31 differential 2-DE spots were defined upon image analysis and 23 proteins were identified to be responsive specifically to arsenate. Of these spots, 18 were unique proteins. These proteins were identified to be phosphate transporters, heat-shock proteins involved in protein refolding, and enzymes participating in carbon and energy metabolism., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2007/011403-0