- Volume 67, Issue 11, 2017
Volume 67, Issue 11, 2017
- Validation List
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- Notification List
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- New taxa
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- Actinobacteria
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Mycobacterium grossiae sp. nov., a rapidly growing, scotochromogenic species isolated from human clinical respiratory and blood culture specimens
A previously undescribed, rapidly growing, scotochromogenic species of the genus Mycobacterium (represented by strains PB739T and GK) was isolated from two clinical sources – the sputum of a 76-year-old patient with severe chronic obstructive pulmonary disease, history of tuberculosis exposure and Mycobacterium avium complex isolated years prior; and the blood of a 15-year-old male with B-cell acute lymphoblastic leukaemia status post bone marrow transplant. The isolates grew as dark orange colonies at 25–37 °C after 5 days, sharing features in common with other closely related species. Analysis of the complete 16S rRNA gene sequence (1492 bp) of strain PB739T demonstrated that the isolate shared 98.8 % relatedness with Mycobacterium wolinskyi . Partial 429 bp hsp65 and 744 bp rpoB region V sequence analyses revealed that the sequences of the novel isolate shared 94.8 and 92.1 % similarity with those of Mycobacterium neoaurum and Mycobacterium aurum , respectively. Biochemical profiling, antimicrobial susceptibility testing, HPLC/gas-liquid chromatography analyses and multilocus sequence typing support the taxonomic status of these isolates (PB739T and GK) as representatives of a novel species. Both isolates were susceptible to the Clinical and Laboratory Standards Institute recommended antimicrobials for susceptibility testing of rapidly growing mycobacteria including amikacin, ciprofloxacin, moxifloxacin, doxycycline/minocycline, imipenem, linezolid, clarithromycin and trimethropin/sulfamethoxazole. Both isolates PB739T and GK showed intermediate susceptibility to cefoxitin. We propose the name Mycobacterium grossiae sp. nov. for this novel species and have deposited the type strain in the DSMZ and CIP culture collections. The type strain is PB739T (=DSM 104744T=CIP 111318T).
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Marmoricola endophyticus sp. nov., an endophytic actinobacterium isolated from Thespesia populnea
A novel endophytic actinobacterium, designated strain 8BXZ-J1T, was isolated from surface-sterilized branches of Thespesia populnea collected from Beilun Estuary Mangrove Forest National Nature Reserve in Guangxi, China, and examined by a polyphasic approach to determine its taxonomic position. Cells of the isolate were Gram-stain-positive, aerobic, non-spore-forming, non-motile and short rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 8BXZ-J1T belonged to the genus Marmoricola , sharing highest similarity with Marmoricola solisilvae DSM 27140T (96.9 %). The isolate grew at 10–35 °C (optimum, 28–30 °C), at pH 6.0–8.0 (optimum, pH 7.0) and in the presence of 0–10 % (w/v) NaCl (optimum, 0–5.0 %). The organism contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, MK-8(H4) as the major menaquinone, and a polar lipid profile including diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown lipids. The major fatty acids of strain 8BXZ-J1T were C18 : 0 10-methyl, iso-C16 : 0 and C16 : 0. The G+C content of the genomic DNA was 68.7 mol%. These data demonstrate that strain 8BXZ-J1T is representative of a novel species of the genus Marmoricola , for which the name Marmoricola endophyticus sp. nov. is proposed. The type strain is 8BXZ-J1T (=KCTC 39789T=CGMCC 1.16067T).
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Corynebacterium gottingense sp. nov., isolated from a clinical patient
More LessA Gram-positive bacterial strain, 99221/2016T, was isolated from blood of a patient with bacteraemia at the Institute of Medical Microbiology, Göttingen, Germany. The strain was rod-shaped with a palisade arrangement of cells, non-spore-forming, non-lipophilic, catalase-positive and oxidase-negative. It grew well at 37 °C on Columbia blood agar and showed good growth under aerobic, microaerophilic and anaerobic conditions. The colonies were white-cream, circular and convex with a shiny, smooth surface. The predominant respiratory quinones were MK-8(H2) and MK-9(H2). The polar lipids profile contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. Two unidentified phospholipids and several unidentified lipids were also detected. The prevalent cellular fatty acids comprised cis-9-octadecenoic acid (C18 : 1 ω9c), hexadecanoic acid (C16 : 0) and pentadecanoic acid (C15 : 0). Corynemycolates with 28–36 carbons in length were present. The whole-cell hydrolysate contained meso-diaminopimelic acid and arabinose, glucose, galactose and ribose as major sugars. Analysis of the 16S rRNA gene sequence identities revealed that the strain is most closely related to Corynebacterium imitans DSM 44264T (98.0 %), Corynebacterium lipophiloflavum DSM 44291T (96.9 %), Corynebacterium afermentans subsp. afermentans DSM 44280T (96.9 %) and Corynebacterium afermentans subsp. lipophilum DSM 44282T (96.8 %). The identity with Corynebacterium diphtheriae DSM 44123T, the type species of the genus, was 94 %. The DNA G+C content was 69.2 mol%. DNA–DNA hybridization with Corynebacterium imitans DSM 44264T revealed a value of 34 %, confirming that the strain represents a novel species. The type strain 99221/2016T (DSM 103494T=JCM 31931T) is proposed to represent a novel species of the genus Corynebacterium with the name Corynebacterium gottingense.
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Ornithinimicrobium flavum sp. nov., isolated from the leaf of Paris polyphylla
A Gram-positive bacterium originating from the surface-sterilized leaf of Paris polyphylla var. yunnanensis (Franch.) was characterized by using a polyphasic approach. The isolate formed yellow, smooth, circular colonies on nutrient agar with 0.2 % starch (NSA). Cells were non-motile, non-sporulating, irregular rods or cocci. Strain CPCC 203535T had the highest 16S rRNA gene sequence similarity to the type strain of Ornithinimicrobium kibberense (96.9 %) and formed the deepest branch in the genus Ornithinimicrobium in the neighbour-joining (NJ) phylogenetic tree based on 16S rRNA gene sequences. The major menaquinones of strain CPCC 203535T were MK-8(H4), MK-8(H2) and MK-8. The peptidoglycan contained ornithine as the diagnostic diamino acid. The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unknown lipid (UL). The major fatty acids iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0 were consistent with the fatty acid patterns reported for members of the genus Ornithinimicrobium . The DNA G+C composition is 71.4 mol%. The results of physiological and biochemical tests allowed phenotypic differentiation of strain CPCC 203535T from its closest phylogenetic species in the genus Ornithinimicrobium . Strain CPCC 203535T represents a novel species of the genus Ornithinimicrobium , for which the name Ornithinimicrobium flavum sp. nov. is proposed, with CPCC 203535T (=NBRC 109452 T=KCTC 29164T) as the type strain.
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Arthrobacter silviterrae sp. nov., isolated from forest soil
A novel actinomycete strain, designated KIS14-16T, was isolated from forest soil in Ongjin county, South Korea and characterized using polyphasic taxonomy. The cells are aerobic, Gram-stain-positive, non-flagellated and short rods. The strain grew in a temperature range of 4–33 °C (optimum, 28–30 °C) and pH range of 5.0–10.0 (optimum, 7.0) and in the presence of 0–5 % (w/v) NaCl (optimum, 0 %). Comparison of 16S rRNA gene sequences showed that strain KIS14-16T is a member of the genus Arthrobacter exhibiting high sequence similarity with A. livingstonensis LI2T (97.7 %), A. cryoconiti Cr6-08T (97.6 %), A. psychrochitiniphilus GP3T (97.4 %), A. stackebrandtii CCM 2783T (97.1 %) and A. globiformis DSM 20124T (96.3 %). DNA–DNA relatedness and phenotypic data distinguished strain KIS14-16T from phylogenetically related type strains. The peptidoglycan type of strain KIS14-16T was A3α, with an interpeptide bridge comprising l-Lys, l-Thr, Gly and l-Ala4. Strain KIS14-16T contained a large amount of MK-9(H2) and relatively small amounts of MK-10(H2) and MK-8(H2). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and dimannosylglyceride. The major fatty acids (>10 %) were anteiso-C15 : 0 and anteiso-C17 : 0. The genomic DNA G+C content was 63.9 mol%. On the basis of these phenotypic, chemotaxonomic and phylogenetic data, strain KIS14-16T should be designated as a representative novel species of the genus Arthrobacter , for which the name Arthrobacter silviterrae sp. nov. is proposed. The type strain is KIS14-16T (=KACC 17303T=DSM 27180T=NBRC 109660T).
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Saccharomonospora colocasiae sp. nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta
A non- Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15 : 1 B, iso-C16 : 1 H, anteiso-C15 : 0 and C15 : 0 2-OH. The DNA G+C content was 69 mol%. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1 %) and Saccharomonospora viridis DSM 43017T (97.1 %) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6 %). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50 %, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora , for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).
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Lechevalieria rhizosphaerae sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Lechevalieria
A novel actinomycete, designated strain NEAU-A2T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-A2T should be assigned to the genus Lechevalieria and forms a distinct branch with its closest neighbour Lechevalieria aerocolonigenes DSM 40034T (99.0 %). Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain NEAU-A2T to the genus Lechevalieria . The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates were galactose, mannose, rhamnose, glucose and ribose. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositolmannoside and two glycolipids. The predominant menaquinones were MK-9(H4) and MK-9(H6). The major fatty acids were iso-C16 : 0, anteiso-C15 : 0, C16 : 1ω7c and anteiso-C17 : 0. The DNA G+C content was 68.2 mol%. The combination of the DNA–DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-A2T could be distinguished from its closest relative. Therefore, it is proposed that strain NEAU-A2T represents a novel species of the genus Lechevalieria , for which the name Lechevalieria rhizosphaerae sp. nov. is proposed. The type strain is NEAU-A2T (=CGMCC 4.7405T=DSM 104541T).
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Nonomuraea cavernae sp. nov., a novel actinobacterium isolated from a karst cave sample
A novel actinobacterial strain, designated SYSU K10005T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain SYSU K10005T was most closely related to the type strains of the genus Nonomuraea , and shared highest sequence similarity of 98.4 % with Nonomuraea candida HMC10T. DNA–DNA hybridization values between the two strains were less than 70 %. The whole-cell hydrolysates of strain SYSU K10005T contained meso-diaminopimelic acid (diagnostic diamino acid), and arabinose, madurose and rhamnose (whole-cell sugars). The major isoprenoid quinone was MK-9(H4), while the major fatty acids were iso-C16 : 0, 10-methyl C17 : 0, C17 : 1ω8c and C17 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, an unidentified lipid, two unidentified ninhydrin-positive phosphoglycolipids and two unidentified phospholipids. The genomic DNA G+C content of strain SYSU K10005T was 64.2 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10005T can be characterized to represent a novel species of the genus Nonomuraea , for which the name Nonomuraea cavernae sp. nov. is proposed. The type strain is SYSU K10005T (=KCTC 39805T=CGMCC 4.7368T).
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Kineococcus terrestris sp. nov. and Kineococcus aureolus sp. nov., isolated from saline sediment
More LessTwo novel actinobacteria, designated YIM 121936T and YIM 121940T, were isolated from alkaline sediment in Yuanjiang, China. The cells of the novel strains were Gram-stain-positive, aerobic, motile, non-spore-forming and coccus-shaped. The two strains both contained meso-diaminopimelic acid as the diagnostic diamino acid. The whole-cell sugars were arabinose, galactose, glucose, mannose and ribose. The predominant menaquinone was MK-9(H2). The polar lipid profile of both strains comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unknown phosphoglycolipid and five unknown phospholipids. The predominant fatty acids of both strains were anteiso-C15 : 0. The genomic DNA G+C contents of strains YIM 121936T and YIM 121940T were 74.7 and 75.2 %, respectively. Strain YIM 121936T was closely related to Kineococcus aurantiacus IFO 15268T (97.19 %), Kineococcus gypseus YIM 121300T (97.00 %) and Kineococcus mangrovi NBRC 110933T (97.00 %). Strain YIM 121940T was closely related to K. aurantiacus IFO 15268T (97.41 %), Kineococcus endophytica KLBMP 1274T (97.18 %), Kineococcus rhizosphaerae RP-B16T (97.09 %), Kineococcus radiotolerans SRS 30216T (97.09 %), K. gypseus YIM 121300T (97.00 %) and K. mangrovi NBRC 110933T (97.00 %). Strain YIM 121936T shared high 16S rRNA gene sequence similarity (99 %) with YIM 121940T. Similarities of two strains with other species of the genus Kineococcus were <97 %. The DNA–DNA hybridization values were below 70 % among all the strains studied. YIM 121936T and YIM 121940T are representatives of two new species in the genus Kineococcus , for which names Kineococcus terreus sp. nov. (type strain YIM 121936T=KCTC 39738T=DSM 102155T) and Kineococcus aureolus sp. nov. (type strain YIM 121940T=KCTC 39739T=DSM 102158T) are proposed, respectively.
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Streptomyces roietensis sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of jasmine rice, Oryza sativa KDML 105
More LessAn endophytic actinobacterium, strain WES2T, was isolated from the stem of a jasmine rice plant collected from a paddy field in Thung Gura Rong Hai, Roi Et province, Thailand. As a result of a polyphasic study, this strain was identified as representing a novel member of the genus Streptomyces . This strain was a Gram-stain-positive, aerobic actinobacterium with well-developed substrate mycelia and forming chains of looped spores. The closest phylogenetic relations, which shared the highest 16S rRNA gene sequence similarity, were Streptomyces nogalater JCM 4799T and Streptomyces lavenduligriseus NRRL-ISP 5487T at 99.1 and 99.0 %, respectively. Chemotaxonomic data, including major fatty acids, cell wall components and major menaquinones, confirmed the affiliation of WES2T to the genus Streptomyces . The data from the phylogenetic analysis, including physiological and biochemical studies and DNA–DNA hybridization, revealed the genotypic and phenotypic differentiation of WES2T from the most closely related species with validly published names. The name proposed for the novel species is Streptomyces roietensis sp. nov. The type strain is WES2T (=DSM 101729=NRRL B-65344).
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Lentzea pudingi sp. nov., isolated from a weathered limestone sample in a karst area
More LessA novel Gram-stain-positive, aerobic bacterium, designated strain DHS C021T, was isolated from a limestone sample collected from the Puding Karst Ecosystem Research Station of Guizhou Province, southwest China. This strain developed branched vegetative mycelia, and its aerial mycelia fragmented into rod-shaped spores. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the whole-cell sugars comprised galactose, ribose and mannose. The respiratory quinone was identified as menaquinone MK-9(H4). The major cellular fatty acids were iso-C14 : 0 and iso-C16 : 0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidyethanolamine, phosphatidylinositol, phosphotidylinositolmannosides and one unidentified phospholipid. The genomic DNA G+C content was 69.8 mol% and 16S rRNA gene sequence analysis showed that the strain belonged to the genus Lentzea and shared highest sequence similarity with Lentzea albida CGMCC 4.1727T (98.8 %) and Lentzea waywayandensis CGMCC 4.1646T (98.5 %). However, it could be distinguished from these reference strains based on the low levels of DNA–DNA relatedness (54.5±2.7 and 41.7±3.2 %, respectively). On the basis of morphological, chemotaxonomic and phylogenetic characteristics, and DNA–DNA hybridization data, strain DHS C021T represents a novel species of the genus Lentzea , for which the name Lentzea pudingi sp. nov. is proposed. The type strain is DHS C021T (=CGMCC 4.7319T=KCTC 39694T).
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- Archaea
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Haloplanus salinarum sp. nov., an extremely halophilic archaeon isolated from a solar saltern
An extremely halophilic archaeal strain SP28T was isolated from the Gomso solar saltern, Republic of Korea. Cells of the new strain SP28T were pleomorphic and Gram stain negative, and produced red-pigmented colonies. These grew in medium with 2.5–4.5 M NaCl (optimum 3.1 M) and 0.05–0.5 M MgCl2 (optimum 0.1 M), at 25–50 °C (optimum 37 °C) and at a pH of 6.5–8.5 (optimum pH 8.0). Mg2+ was required for growth. A concentration of at least 2 M NaCl was required to prevent cell lysis. Polar lipids included phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one glycolipid chromatographically identical to sulfated mannosyl glucosyl diether. 16S rRNA and rpoB′ gene sequence analyses showed that strain SP28T is closely related to Haloplanus ruber R35T (97.3 and 94.1 %, 16S rRNA and rpoB′ gene sequence similarity, respectively), Haloplanus litoreus GX21T (97.0 and 92.1 %), Haloplanus salinus YGH66T (96.0 and 91.9 %), Haloplanus vescus RO5-8T (95.9 and 90.9 %), Haloplanus aerogenes TBN37T (95.6 and 90.3 %) and Haloplanus natans RE-101T (95.3 and 89.8 %). The DNA G+C content of the novel strain SP28T was 66.2 mol%, which is slightly higher than that of Hpn. litoreus GX21T (65.8 mol%) and Hpn. ruber R35T (66.0 mol%). DNA–DNA hybridization values betweenHpn. ruber R35T and strain SP28T and between Hpn. litoreus GX21T and strain SP28T were about 24.8 and 20.7 %, respectively. We conclude that strain SP28T represents a novel species of the genus Haloplanus and propose the name Haloplanus salinarum sp. nov. The type strain is SP28T (=JCM 31424T=KCCM 43210T).
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- Bacteroidetes
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Bacteroides koreensis sp. nov. and Bacteroides kribbi sp. nov., two new members of the genus Bacteroides
Three bacterial isolates from human faeces, YS-aM39T, R2F3-3-3T and R2F3-5-1, were characterized as Gram-negative, strictly anaerobic, non-spore-forming, non-motile, and rod-shaped. Isolate YS-aM39T formed a distinct line of descent, showing greatest 16S rRNA gene sequence relatedness with R2F3-3-3T (97.5 %), R2F3-5-1 (97.5 %), Bacteroides ovatus (98.8 %) and Bacteroides xylanisolvens (97.2 %). Isolates R2F3-3-3T and R2F3-5-1 also formed a distinct line of descent, sharing greatest 16S rRNA gene sequence relatedness with B. ovatus (98.2 %) and B. xylanisolvens (97.2 %). The DNA G+C content of YS-aM39T was 44.8 mol%, that of R2F3-3-3T was 42.4 mol% and that of R2F3-5-1 was 42.6 mol%. The respiratory quinone of all three isolates was menaquinone MK-10. Polar lipid analysis identified phosphatidylethanolamine as the major lipid. The predominant fatty acids in all three isolates were anteiso-C15 : 0, iso-C15 : 0, C16 : 0 3-OH and iso-C17 : 0 3-OH. The major end products of glucose fermentation were acetic acid, lactic acid and formic acid. DNA–DNA hybridization data indicated that two isolates, YS-aM39T and R2F3-3-3T, represent a species distinct from B. ovatus and B. xylanisolvens . Finally, in this study, the two isolates represented two new species in the genus Bacteroides , for which we propose the names Bacteroides koreensis sp. nov. (type strain, YS-aM39T=KCTC 15520T=JCM 31393T) and Bacteroides kribbi sp. nov. (type strain, R2F3-3-3T=KCTC 15460T=JCM 31391T).
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Pseudomonas tarimensis sp. nov., an endophytic bacteria isolated from Populus euphratica
An endophytic bacterium, MA-69T, was isolated from the storage liquid in the stems of Populuseuphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain MA-69T was found to be short rod-shaped, Gram-stain-negative, non-spore-forming, aerobic and motile by means of a monopolar flagellum. According to phylogenetic analysis based on 16S rRNA gene sequences, strain MA-69T was assigned to the genus Pseudomonas with highest 16S rRNA gene sequence similarity of 97.5 % to Pseudomonas azotifigens JCM 12708T, followed by Pseudomonas matsuisoli JCM 30078T (97.5 %), Pseudomonas balearica DSM 6083T (97.1 %), Azotobacter salinestris ATCC 49674T (96.1 %) and Pseudomonas indica DSM 14015T (95.9 %). Analysis of strain MA-69T based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the isolate to be distinctly delineated from species of the genus Pseudomonas . The DNA G+C content of strain MA-69T was 64.1 mol%. DNA–DNA hybridization with Pseudomonas azotifigens JCM 12708T, Pseudomonas matsuisoli JCM 30078T and Pseudomonas balearica DSM 6083T revealed 62.9, 60.1 and 49.0 % relatedness, respectively. The major fatty acids in strain MA-69T were summed feature 3 (25.7 %), summed feature 8 (24.0 %), C19 : 0cyclo ω8c (19.9 %), C16 : 0 (14.6 %) and C12 : 0 (6.3 %). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Q-9 was the major quinone in strain MA-69T. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain MA-69T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas tarimensis sp. nov. is proposed. The type strain is MA-69T (=CCTCC AB 2013065T=KCTC 42447T).
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Flavobacterium chuncheonense sp. nov. and Flavobacterium luteum sp. nov., isolated from a freshwater lake
More LessTwo Gram-staining-negative, orange coloured, non-motile, rod-shaped bacterial strains, designated strains IMCC26013T and IMCC26026T, were isolated from a freshwater sample collected from Lake Soyang in Korea. The 16S rRNA gene-based phylogenetic analyses showed that both strains belonged to the genus Flavobacterium and that strains IMCC26013T and IMCC26026T were most closely related to Flavobacterium psychrophilum (96.5 %) and Flavobacterium myungsuense (97.7 %), respectively. DNA G+C contents of strains IMCC26013T and IMCC26026T were 37.8 and 33.7 mol%, respectively. DNA–DNA relatedness between strain IMCC26026T and F. myungsuense HMD1033T was 56.4 %, showing a novel species status of strain IMCC26026T. Major fatty acid constituents (>10 %) of strain IMCC26013T were iso-C15 : 1 G, C15 : 1 ω6c, C17 : 1 ω6c and summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and those of strain IMCC26026T were iso-C15 : 0, anteiso-C15 : 0 and summed feature 3. Respiratory quinone detected in the two strains was MK-6. Both strains contained phosphatidylethanolamine as a major polar lipid. On the basis of these results, strains IMCC26013T and IMCC26026T were considered to represent novel species in the genus Flavobacterium , for which the names Flavobacterium chuncheonense (type strain IMCC26013T=KCTC 52573T=NBRC 112526T), and Flavobacterium luteum (type strain IMCC26026T=KCTC 52572T=NBRC 112527T) are proposed, respectively.
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Flavobacterium dispersum sp. nov., isolated from a freshwater spring
More LessA novel bacterial strain MVW-23T was isolated from a freshwater spring in Taiwan. The strain was Gram-staining-negative, strictly aerobic, motile by gliding, rod-shaped and formed translucent yellow colonies. Optimal growth occurred at 20–30 °C, pH 7.0, and in the presence of 0.5–1 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-23T belonged to the genus Flavobacterium and showed the highest levels of sequence similarity with respect to Flavobacterium denitrificans ED5T (97.3 %), Flavobacterium kyungheense THG-107T (97.2 %) and Flavobacterium defluvii EMB117T (97.0 %). Strain MVW-23T contained iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C17 : 0 3-OH as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, three uncharacterized aminophospholipids and one uncharacterized phospholipid. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 39.9 mol%. The DNA–DNA hybridization value for strain MVW-23T with F. denitrificans DSM 15936T, F. kyungheense LMG 26575T and F. defluvii DSM 17963T was less than 35 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain MVW-23T should be classified as a novel species of the genus Flavobacterium , for which the name Flavobacterium dispersum sp. nov. is presented. The type strain is MVW-23T (=BCRC 80978T=LMG 29558T=KCTC 52234T).
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Edaphobaculum flavum gen. nov., sp. nov., a member of family Chitinophagaceae, isolated from grassland soil
More LessA yellow-coloured, Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterium, designated 1-116T, was isolated from Erdos grassland soil of Inner Mongolia, PR China. Phylogenetic analysis based on 16S rRNA genes showed that strain 1-116T was a member of family Chitinophagaceae and exhibited the highest similarities to Taibaiella koreensis THG-DT86T (90.1 %) and Flavihumibacter solisivae 3-3T (90.0 %), while the similarities to the other Chitinophagaceae type strains were lower than 90.0 %. Strain 1-116T grew at 16–33 °C (optimum 28 °C), pH 6.0–9.0 (optimum 7.0–8.0) and 0–0.5 % NaCl (w/v; optimum without NaCl). A flexirubin-type pigment was present. The DNA G+C content was 43.2 mol% and the only quinone present was menaquinone-7. The only polyamine detected was sym-homospermidine [30.7 µmol (g dry weight)−1] and the predominant fatty acids were iso-C15 : 0 (20.8 %), iso-C15 : 1 G (25.1 %), summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I; 13.8 %) and iso-C17 : 0 3-OH (13.2 %). The major polar lipids were phosphatidylethanolamine, three unidentified lipids, an unidentified aminophospholipid and an unidentified glycolipid. On the basis of the polyphasic analyses, strain 1-116T represents a novel genus and species in the family Chitinophagaceae , for which the name Edaphobaculum flavum gen. nov., sp. nov., is proposed. The type strain of Edaphobaculum flavum is 1-116T (=CCTCC AB 2017054=KCTC 52843).
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Dokdonia lutea sp. nov., isolated from Sargassum fulvellum seaweed
More LessA non-motile, yellow-coloured and rod-shaped bacterium, designated strain SFD34T, was isolated from seaweed collected from the South Sea, Republic of Korea. Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. Strain SFD34T contained MK-6 and phosphatidylethanolamine as the only isoprenoid quinone and major polar lipid, respectively. The DNA G+C content was 35 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain SFD34T formed evolutionary lineage within the radiation enclosing the members of the genus Dokdonia with Dokdonia pacifica SW230T (96.86 % sequence similarity) as its nearest neighbour. A number of phenotypic characteristics distinguished strain SFD34T from related members of the genus Dokdonia . On the basis of the evidence presented in this study, a novel species, Dokdonia lutea sp. nov., is proposed for strain SFD34T (=KCTC 52269T=JCM 31795T).
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Arenibacter antarcticus sp. nov., isolated from marine sediment
More LessA strictly aerobic, Gram-stain-negative, pale-golden, rod-shaped bacterium, designated as R18H21T, was isolated from marine sediment collected from the Ross Sea, Antarctica. Strain R18H21T grew at 4–40 °C (optimum 25 °C), at pH 6.3–9.2 (optimum 7.5–8.5) and in 0.5–6 % (w/v) NaCl (optimum 2 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain R18H21T belonged to the genus Arenibacter , with the highest similarity to two type strains, Arenibacter latericius KMM 426T (96.6 %) and Arenibacter certesii KMM 3941T (96.6 %), and lower similarities (95.2–95.9 %) to five other members of the genus Arenibacter . The major fatty acids were iso-C17 : 0 3-OH, Summed Feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c), iso-C15 : 0, iso-C15 : 1 G. The major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified phospholipid. The respiratory quinone of strain R18H21T was menaquinone-6. The DNA G+C content was 40.0 mol%. Based on phylogenetic, physiological and chemotaxonomic features, strain R18H21T has been classified as a novel species in the genus Arenibacter , for which the name Arenibacter antarcticus sp. nov. is proposed. The type strain of the novel species is R18H21T (=GDMCC 1.1159T=KCTC 52924T).
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Tenacibaculum aestuariivivum sp. nov., isolated from a tidal flat
More LessA Gram-stain-negative, aerobic, non-motile, non-spore-forming bacterial strain, designated JDTF-79T, was isolated from a tidal flat in Jindo, an island of South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain JDTF-79T grew optimally at 25 °C and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain JDTF-79T fell within the clade comprising the type strains of species of the genus Tenacibaculum , clustering with the type strains of Tenacibaculum dicentrarchi , Tenacibaculum ovolyticum , ‘ Tenacibaculum haliotis’ and Tenacibaculum soleae . The novel strain exhibited highest 16S rRNA gene sequence similarity (98.3 %) to the type strain of T. dicentrarchi and sequence similarities of 93.5–96.9 % to the type strains of the other species of the genus Tenacibaculum . Strain JDTF-79T contained MK-6 as the predominant menaquinone and anteiso-C15 : 0, iso-C15 : 0 3-OH and iso-C15 : 0 as the major fatty acids. The major polar lipids of strain JDTF-79T were phosphatidylethanolamine, one unidentified lipid and one unidentified aminophospholipid. The DNA G+C content of strain JDTF-79T was 30.3 mol%. Strain JDTF-79T had a mean DNA–DNA relatedness value of 19 % with the type strain of T. dicentrarchi . The differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain JDTF-79T is separated from other recognized species of the genus Tenacibaculum . On the basis of the data presented, strain JDTF-79T is considered to represent a novel species of the genus Tenacibaculum , for which the name Tenacibaculum aestuariivivum sp. nov. is proposed. The type strain is JDTF-79T (=KCTC 52980T=NBRC 112903T).
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Sphingobacterium humi sp. nov., isolated from soil
More LessA Gram-stain-negative, facultatively aerobic bacterium, designated strain D1T, was isolated from soil in South Korea. Cells of strain D1T were non-motile rods with oxidase- and catalase-positive activities. Growth was observed at 15–40 °C (optimum, 30–37 °C), at pH 5.5–9.0 (optimum, pH 7.0–8.0) and in the presence of 0.0–5.0 % (w/v) NaCl (optimum, 0.0–1.0 %). The only respiratory quinone detected was menaquinone 7 (MK-7), and iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c) were identified as the major fatty acids. Phosphatidylethanolamine was the major polar lipid, and two unidentified glycophospholipids and four unidentified lipids were also detected as minor polar lipids. Sphingolipids, a typical chemotaxonomic feature of the genus Sphingobacterium , were detected. The G+C content of the genomic DNA was 43.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D1T formed a phyletic lineage with Sphingobacterium hotanense XH4T within the genus Sphingobacterium . Strain D1T was most closely related to S. hotanense XH4T (98.1 % 16S rRNA gene sequence similarity) and Sphingobacterium cellulitidis R-53603T (97.2 %), and the DNA–DNA relatedness level between strain D1T and the type strain of S. cellulitidis was 43.1±0.7 %. Based on the phenotypic, chemotaxonomic and molecular features, strain D1T clearly represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium humi sp. nov. is proposed. The type strain is D1T (=KACC 18595T=JCM 31225T).
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Puia dinghuensis gen. nov., sp. nov., isolated from monsoon evergreen broad-leaved forest soil
More LessA Gram-stain-negative, rod-shaped, aerobic, non-motile bacterial strain, 4GSH07T, was originally isolated from the monsoon evergreen broad-leaved forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31′ E 23° 10′ N). It grew optimally at 28–33 °C and pH 4.0. The 16S rRNA gene sequence analysis showed that strain 4GSH07T had the highest sequence similarity of 94.0 % to Parasegetibacter terrae JCM 19942T, and formed an independent lineage separable from other described genera of the family Chitinophagaceae . The main fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0 3-OH, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and C15 : 0 2-OH. The organism contained MK-7 as the predominant respiratory quinone, and the total DNA G+C content was 50.3 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed consistently that strain 4GSH07T represents a novel species of a novel genus of the family Chitinophagaceae , for which the name Puia dinghuensis gen. nov., sp. nov., is proposed, with 4GSH07T (=CGMCC 1.15448T=LMG 29214T) as the type strain.
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Flavobacterium limi sp. nov., isolated from forest mud
More LessA Gram-stain-negative, aerobic, non-motile, yellow and rod-shaped bacterial strain was isolated from forest mud located at Kyung Hee University, South Korea. Strain THG-AG6.4T grew at 10–35 °C, pH 6.0–9.5 and in the presence of 0–1.5 % (w/v) NaCl. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain THG-AG6.4T was most closely related to Flavobacterium gyeonganense HME 7524T (97.66 %), Flavobacterium defluvii EMB 117T (96.93 %) and Flavobacterium arsenitoxidans S2-3HT (96.80 %). The DNA G+C content of strain THG-AG6.4T was 30.2 mol%. The DNA–DNA relatedness values between strain THG-AG6.4T and its closest phylogenetic neighbour, F. gyeonganense HME 7524T, were below 61.0 %. The predominant quinone of strain THG-AG6.4T was MK-6. The major polar lipids were phosphatidylethanolamine, an unidentified phospholipid, five unidentified glycolipids, phosphatidylserine, an unidentified lipid, an unidentified aminophospholipid, two unidentified aminolipids and two unidentified aminoglycolipids. The major fatty acids were C16 : 0, C18 : 0 10-methyl, summed feature 3 and C18 : 1ω9c. The major polyamine was homospermidine. On the basis of the phenotypic, genotypic and phylogenetic characterization of strain THG-AG6.4T, it is concluded that the isolate represents a novel species of the genus Flavobacterium , for which the name Flavobacterium limi sp. nov. is proposed, with THG-AG6.4T as the type strain (=KACC 18851T=CGMCC 1.16060T).
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Sediminibacterium roseum sp. nov., isolated from sewage sediment
More LessA novel bacterial strain, designated SYL130T, was isolated from the sewage sediment of a park in Busan, Korea. The strain was aerobic, producing orange colonies on R2A agar. Cells were single, Gram-stain-negative rods and were motile by gliding. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain SYL130T was most closely related to Sediminibacterium aquarii JCM 31013T (96.1 %). The major fatty acids (>5 % of the total) of strain SYL130T were iso-C15 : 0 (28.3 %), iso-C15 : 1 G (23.2 %), iso-C17 : 0 3-OH (9.6 %), anteiso-C15 : 0 (5.9 %) and iso-C16 : 0 3-OH (5.6 %). The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, two unidentified aminophospholipids and six unidentified polar lipids. The predominant respiratory quinone was MK-7. The DNA G+C content was 47.8 mol%. Strain SYL130T had clearly differential characteristics to related species including the temperature and pH ranges for growth, and being positive for l-arabinose and maltose, and negative for α-galactosidase activity. On the basis of phenotypic, chemotaxonomic and genotypic analyses, strain SYL130T represents a novel species of the genus Sediminibacterium , for which the name Sediminibacterium roseum sp. nov., is proposed. The type strain Sediminibacterium roseum is SYL130T (=KCTC 52860T=CCTCC AB 2017082T).
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Parapedobacter defluvii sp. nov., isolated from the sewage treatment packing of a coking chemical plant
Strain WY-1T, a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium, was isolated from the sewage treatment packing of a coking chemical plant. Strain WY-1T grew over a temperature range of 15–45 °C (optimum, 30–37 °C), a pH range of 5.5–11.0 (optimum, pH 6.5–7.0) and an NaCl concentration range of 0–3 % (w/v; optimum, 0 %). 16S rRNA gene sequence analysis showed that strain WY-1T was closely related to Parapedobacter indicus RK1T with the highest sequence similarity of 96.0 %. The predominant cellular fatty acids of the novel strain were iso-C15 : 0, summed feature 3(C16 : 1ω6c and/or C16 : 1ω7c), iso-C17 : 0 3-OH, iso-C17 : 1ω9c, iso-C15 : 0 3-OH and C16 : 0. The respiratory quinone of the cells was menaquinone 7 (MK-7). The main polar lipid was phosphatidylethanolamine, an unidentified phospholipid, two unidentified aminolipids and two unknown lipids. The G+C content of the DNA was 47.1 mol%. Chemotaxonomic characteristics and phylogenetic analyses revealed that strain WY-1T belonged to the genus Parapedobacter . Strain WY-1T showed a range of phenotypic characteristics that differentiated it from species of the genus Parapedobacter with validly published names, including its assimilation from carbon sources, enzyme activities and having a wider pH range for growth. Based on these results, it is concluded that strain WY-1T represents a novel species of the genus Parapedobacter , for which the name Parapedobacter defluvii sp. nov. is proposed. The type strain is WY-1T (=NBRC 112611T=CGMCC 1.15342T).
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Hymenobacter gummosus sp. nov., isolated from a spring
More LessStrain ANT-18T, isolated from a water sample taken from the Longtoushan Spring in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain ANT-18T were Gram-stain-negative, aerobic, motile by gliding rods that were surrounded by a thick capsule and it formed pink colonies. Growth occurred at 20–37 °C (optimum, 25 °C), at pH 6–8 (optimum, pH 7) and with 0–1 % NaCl (w/; optimum, 0 %). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain ANT-18T belongs to the genus Hymenobacter and showed the highest levels of sequence similarity to Hymenobacter ocellatus Myx 2105T (97.8 %) and Hymenobacter paludis KBP-30T (97.7 %). Strain ANT-18T contained iso-C15 : 0, anteiso-C15 : 0, C18 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, one uncharacterized aminolipid, two uncharacterized aminophospholipids, two uncharacterized glycolipids and seven uncharacterized lipids. The major polyamine was homospermidine. The genomic DNA G+C content of strain ANT-18T was 63.6 mol%. The DNA–DNA relatedness values of strain ANT-18T with respect to H. ocellatus Myx 2105T and H. paludis KBP-30T were less than 35 %. On the basis of the phylogenetic inference and phenotypic data, strain ANT-18T is recognized to be a representative of a novel species within the genus Hymenobacter. The name Hymenobacter gummosus sp. nov. is proposed, with strain ANT-18T (=BCRC 80966T=LMG 29477T=KCTC 52166T) as the type strain.
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Polaribacter tangerinus sp. nov., isolated from sediment in a sea cucumber culture pond
More LessA Gram-stain-negative, aerobic, non-motile, coccoid, ovoid or rod-shaped, and orange-pigmented bacterial strain, designated WS2-14T, was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, PR China (122° 14′ E, 36° 54′ N). Strain WS2-14T grew optimally at 28 °C and pH 7.0–7.5, and was able to tolerate salt concentrations of 0.5–6.0 % (w/v) NaCl. Strain WS2-14T was characterized chemotaxonomically as possessing menaquinone-6 (MK-6) as the major respiratory quinone, as well as iso-C13 : 0, iso-C15 : 0 3-OH and iso-C15 : 0 as the predominant fatty acids. The DNA G+C content of strain WS2-14T was 31.2 mol%. The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and three unidentified lipids. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain WS2-14T was phylogenetically related to members of the genus Polaribacter and was closely related to Polaribacter haliotis , Polaribacter atrinae and Polaribacter sejongensis with 97.7, 97.4 and 97.0 % sequence similarities, respectively. On the basis of its phenotypic and chemotaxonomic properties, as well as phylogenetic analyses, strain WS2-14T was considered to represent a novel species of the genus Polaribacter , for which the name Polaribacter tangerinus sp. nov. is proposed. The type strain is WS2-14T (=KCTC 52275T=MCCC 1H00163T).
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Kordia zosterae sp. nov., isolated from the seaweed, Zostera marina
More LessA Gram-stain-negative, gliding and rod shaped bacterium, designated strain ZO2-23T was isolated from a seaweed sample collected from the West Sea, Republic of Korea. Cells are catalase-negative and oxidase-positive. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain ZO2-23T forms an independent lineage within the genus Kordia . Strain ZO2-23T was related to Kordia ulvae SC2T (98.0 %, 16S rRNA gene sequence similarity) and K. antarctica IMCC3317T (97.9 %). The major fatty acids of strain ZO2-23T were iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 and iso-C15 : 0 3-OH. The only isoprenoid quinone of the isolate was menaquinone-6. The DNA G+C content of strain ZO2-23T was 31.7 mol%. Phenotypic characteristics distinguished strain ZO2-23T from the related species of the genus Kordia . On the basis of the evidences presented in this study, novel species, Kordia zosterae sp. nov., is proposed for strain ZO2-23T (=KCTC 52268T=JCM 31799T).
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Sabulilitoribacter arenilitoris sp. nov., isolated from seashore sand
More LessA Gram-stain-negative and non-motile bacterial strain that formed rods and orange-pigmented colonies, designated HMF6543T, was isolated from sand of seashore on the South Sea, Republic of Korea. Strain HMF6543T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HMF6543T belonged to the genus Sabulilitoribacter . The most closest related species was Sabulilitoribacter multivorans M-M16T (96.7 %, sequence similarity). Strain HMF6543T contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain HMF6543T were phosphatidylethanolamine, two unidentified aminolipids, one unidentified glycolipid and three unidentified lipids. The DNA G+C content of strain HMF6543T was 31.9 mol%. On the basis of the evidence presented in this study, strain HMF6543T represents a novel species of the genus Sabulilitoribacter , for which the name Sabulilitoribacter arenilitoris sp. nov. is proposed. The type strain is HMF6543T (=KCTC 52401T=NBRC 112674T).
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Sphingobacterium tabacisoli sp. nov., isolated from a tobacco field soil sample
A Gram-staining-negative, non-motile and rod-shaped bacterium, designated strain h337T, was isolated from an arable soil sample of a tobacco field in Kunming, south-west China. The cells showed oxidase-positive and catalase-positive reactions. Growth was observed at 10–35 °C, at pH 6.0–9.0 and in the presence of up to 3 % (w/v) NaCl, with optimal growth at 30 °C, pH 7.0 and with 1–2 % (w/v) NaCl. The predominant isoprenoid quinone was MK-7. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The cellular polar lipids contained phosphatidylethanolamine, sphingophospholipid, four unidentified phospholipids, five unidentified lipids and three unidentified aminophospholipids. The genomic DNA G+C content was 41.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain h337T should be assigned to the genus Sphingobacterium . 16S rRNA gene sequence similarity analysis showed that strain h337T was most closely related to ‘ Sphingobacterium yamdrokense ’ 3-0-1 (98.8 %) and Sphingobacterium yanglingense CCNWSP36-1T (98.5 %) and shared less than 97 % similarity with other species of the genus Sphingobacterium . DNA–DNA hybridization data indicated that the isolate represented a novel genomic species belonging to the genus Sphingobacterium . The characteristics determined in this polyphasic taxonomic study indicated that strain h337T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium tabacisoli sp. nov. (type strain h337T=KCTC 52298T=CCTCC AB 2017155T) is proposed.
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Pedobacter solisilvae sp. nov., isolated from forest soil
More LessThe bacteria strain EN12T was isolated from forest soil in the Republic of Korea. The cells were Gram-negative, non-motile and rod-shaped, and the strain was strictly aerobic. Phylogenetic analysis of its 16S rRNA gene sequences showed that strain EN12T belonged to the class Sphingobacteriia of the phylum Bacteroidetes , and its closest relative is Pedobacter namyangjuensis 5G38T, with a sequence similarity of 95.5 %. The average DNA sequence similarity from validly described species within the genus Pedobacter was 92.5±1.3 %. Chemotaxonomic data including major ubiquinones (menaquinone-7), polar lipids (phosphatidylethanolamine and sphingolipid) and fatty acids (iso-C15 : 0, iso-C17 : 0 3-OH, and C16 : 1 ω6c/C16 : 1 ω7c) also supported an affiliation of strain EN12T with the genus Pedobacter . Genotypic and phenotypic differentiation of strain EN12T from six published Pedobacter species was revealed through DNA–DNA relatedness and physiological/biochemical tests. Results of these phenotypic, phylogenetic and chemotaxonomic analyses indicated that strain EN12T is a novel species in the genus Pedobacter , for which we propose the name Pedobacter solisilvae sp. nov. (=KCTC 42612T=LMG 28820T).
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Hymenobacter cavernae sp. nov., isolated from a karst cave
More LessA novel Gram-stain-negative, rod-shaped, non-motile and red-pigmented bacterial strain, designated K1E01-27T, was isolated from an animal excrement sample which was found in a karst cave located in Guizhou province, China. The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K1E01-27T represented a member of the genus Hymenobacter within the family Cytophagaceae of the phylum Bacteroidetes . Strain K1E01-27T was most closely related to Hymenobacter algoricola VUG-A23aT, with 95.1 % 16S rRNA gene sequence similarity. Growth of strain K1E01-27T occurred at 4–35 °C, at pH 5–9 and in the presence of 0–0.5 % (w/v) NaCl. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 1ω5c, summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 4 (comprising iso-C17 : 1 I/anteiso-C17 : 1 B). The major isoprenoid quinone was menaquinone 7 (MK-7). The major polar lipids were phosphatidylethanolamine, five unidentified aminophospholipids, three unidentified glycolipids, two unidentified phospholipids and one unidentified polar lipid. The DNA G+C content of the genomic DNA was 54.9 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain K1E01-27T is considered to represent a novel species of the genus Hymenobacter , for which the name Hymenobacter cavernae sp. nov. is proposed. The type strain is K1E01-27T (=CGMCC 1.15197T=NBRC 112610T).
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Seonamhaeicola marinus sp. nov., isolated from marine algae
More LessA Gram-stain-negative, facultatively anaerobic, rod-shaped, non-flagellated and non-gliding bacterium, designated strain B011T, was isolated from marine algae Gracilaria blodgettii collected from the coast of Lingshui county, Hainan, China. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain B011T, Seonamhaeicola algicola (96.8 % 16S rRNA gene sequence similarity) and Seonamhaeicola aphaedonensis (95.0 %) belonged to the same clade. Optimal growth occurred in the presence of 3.0 % (w/v) NaCl, at pH 6.5–7.5 and at 28 °C. Menaquinone-6 (MK-6) was the predominant respiratory quinone. The major fatty acids (>10 %) were iso-C15 : 1 G (23.4 %) and iso-C15 : 0 (22.8 %). The major polar lipids were phosphatidylethanolamine, one aminolipid and three unknown lipids. The DNA G+C content of strain B011T was 33.9 mol%. On the basis of polyphasic analysis, strain B011T is considered to represent a novel species in the genus Seonamhaeicola , for which the name Seonamhaeicola marinus sp. nov. is proposed. The type strain is B011T (=MCCC 1H00146T=NBRC 112333T).
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- Firmicutes and Related Organisms
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Streptococcus ovuberis sp. nov., isolated from a subcutaneous abscess in the udder of a sheep
One unidentified, Gram-stain-positive, catalase-negative coccus-shaped organism was recovered from a subcutaneous abscess of the udder of a sheep and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolate was tentatively assigned to the genus Streptococcus , although the organism did not appear to match any recognized species. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus Streptococcus and showed that the nearest phylogenetic relatives of the unknown coccus corresponded to Streptococcus moroccensis and Streptococcus cameli (95.9 % 16S rRNA gene sequence similarity). The sodA sequence analysis showed less than 89.3 % sequence similarity with the currently recognized species of the genus Streptococcus . The novel bacterial isolate was distinguished from close relatives of the genus Streptococcus by using biochemical tests. A mass spectrometry profile was also obtained for the novel isolate using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as a representative of a novel species of the genus Streptococcus , Streptococcus ovuberis sp. nov. The type strain of Streptococcus ovuberissp. nov. is VB15-00779T (=CECT 9179T=CCUG 69612T).
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Paenibacillus arcticus sp. nov., isolated from Arctic soil
A Gram-positive, endospore-forming, strictly aerobic and rod-shaped bacterium, designated strain MME2_R6T, was isolated from Arctic soil, and it was identified by using a polyphasic taxonomic approach. This strain was psychrotolerant, growing at 4‒24 °C. 16S rRNA gene sequence analysis showed that strain MME2_R6T was closest to Paenibacillus swuensis DY6T, with 93.9 % similarity. However, in phylogenetic analysis based on the 16S rRNA gene sequence, strain MME2_R6T showed that it clustered with Paenibacillus contaminans CKOBP-6T and the sequencing similarity between the two species was 93.7 %. Its major cellular fatty acid was anteiso-C15 : 0, like other Paenibacillus species. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The predominant isoprenoid quinone was menaquinone-7. The diagnostic diamino acid in the cell wall was meso-diaminopimelic acid. The genomic DNA G+C content was 44.2 mol%. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, a novel species, Paenibacillus arcticus sp. nov., is proposed. The type strain is MME2_R6T (=JCM 30981T=PAMC 28731T).
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Lactococcus petauri sp. nov., isolated from an abscess of a sugar glider
A strain of lactic acid bacteria, designated 159469T, isolated from a facial abscess in a sugar glider, was characterized genetically and phenotypically. Cells of the strain were Gram-stain-positive, coccoid and catalase-negative. Morphological, physiological and phylogenetic data indicated that the isolate belongs to the genus Lactococcus . Strain 159469T was closely related to Lactococcus garvieae ATCC 43921T, showing 95.86 and 98.08 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively. Furthermore, a pairwise average nucleotide identity blast (ANIb) value of 93.54 % and in silico DNA–DNA hybridization value of 50.7 % were determined for the genome of strain 159469T, when compared with the genome of the type strain of Lactococcus garvieae . Based on the data presented here, the isolate represents a novel species of the genus Lactococcus , for which the name Lactococcus petauri sp. nov. is proposed. The type strain is 159469T (=LMG 30040T=DSM 104842T).
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Desertibacillus haloalkaliphilus gen. nov., sp. nov., isolated from a saline desert
Two Gram-stain-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains KJ1-10-99T and KJ1-10-93, were isolated from a saline desert of Little Rann of Kutch, Gujarat, India. Analysis of 16S rRNA gene sequences revealed that the isolates belonged to the family Bacillaceae and were closely related to each other with 16S rRNA gene sequence similarity of 99.9 %. However, these two isolates formed a novel phylogenetic branch within this family. Both strains were aerobic, catalase and oxidase positive, and could grow optimally at 37 °C and pH 9. Further, strains KJ1-10-99T and KJ1-10-93 grew optimally at a NaCl concentration of 7.5 and 15 % (w/v), respectively. Both strains shared highest sequence similarity with Fermentibacillus polygoni IEB3T (96.90 %) followed by Bacillus nanhaiisediminis NH3T (96.3 %) and Bacillus alkalinitrilicus ANL-iso4T (96.3 %). The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17:0, C16 : 0, and iso-C15 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol in both strains. The predominant isoprenoid quinone was MK-7 in both the strains. The peptidoglycan contained meso-diaminopimelic acid (meso-DAP) as the diagnostic diamino acid. The DNA G+C content of strains KJ1-10-99T and KJ1-10-93 were 48.7 and 48.9 mol% respectively. Both strains could be distinguished from closest phylogenetic neighbours based on a number of phenotypic properties. On the basis of polyphasic taxonomic analysis and phylogenetic data, we conclude that the strains KJ1-10-99T (=LMG 29918T=KCTC 33878T) and KJ1-10-93 (=LMG 29919=KCTC 33877) represent a novel species of a new genus in the family Bacillaceae , order Bacillales , for which the name Desertibacillus haloalkaliphilus gen. nov., sp. nov. is proposed.
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Bacillus solisilvae sp. nov., isolated from forest soil
A novel Gram-stain-positive, motile, endospore-forming, rod-shaped bacterial strain, NEAU-cbsb5T, was isolated from forest soil from Changbai Mountain, Heilongjiang Province, China. The isolate grew at 15–40 °C (optimum 30 °C), at pH 6.0–8.0 (optimum pH 7.0) and in the presence of up to 4 % (w/v) NaCl, although NaCl was not required for growth. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-cbsb5T formed a distinct lineage within the genus Bacillus and was most closely related to Bacillus acidiceler DSM 18954T (99.1 % similarity) and Bacillus luciferensis JCM 12212T (99.0 %). 16S rRNA gene sequence similarity to sequences of the type strains of other Bacillus species was less than 96.0 %. Average nucleotide identity (ANI) values between NEAU-cbsb5T and its most closely related species were 78.72–84.75 % by ANIm, ANIb and OrthoANIu analysis. The in silico DNA–DNA hybridization values between strain NEAU-cbsb5T and its close relatives B. acidiceler DSM 18954T and B. luciferensis JCM 12212T were both 23.80 %, again indicating they belong to different taxa. The major cellular fatty acids of NEAU-cbsb5T were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unknown aminophospholipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinones were MK-7 and MK-6. The genomic DNA G+C content was 33.0 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain NEAU-cbsb5T was classified as a representative of a novel species in the genus Bacillus , for which the name Bacillus solisilvae sp. nov. is proposed. The type strain is NEAU-cbsb5T (=CGMCC 1.14993T=DSM 100485T).
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Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus)
A novel Gram-stain-positive, motile, endospore-forming, rod-shaped bacterium, designated strain NEAU-3TGS17T, was isolated from the head of an ant (Lasius fuliginosus). The isolate grew at 0–35 °C (optimum 28–30 °C), at pH 6.0–11.0 (optimum pH 7.0–8.0) and with 0–6 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-3TGS17T belonged to the genus Psychrobacillus . Sequence similarities between strain NEAU-3TGS17T and members of the genus Psychrobacillus with validly published names ( Psychrobacillus psychrotolerans DSM 11706T, Psychrobacillus insolitus DSM 5T, Psychrobacillus psychrodurans DSM 11713T and Psychrobacillus soli NBRC 110600T) were 98.4–99.1 %. DNA–DNA relatedness values between strain NEAU-3TGS17T and its closest relatives were below 70 %. The major cellular fatty acids (>5 %) were iso-C14 : 0, anteiso-C15 : 0, C16 : 0 and iso-C15 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The cell-wall peptidoglycan type was A4β with ornithine as the diamino acid and the predominant menaquinones were MK-8 and some MK-7. The DNA G+C content was 35.8 mol%. On the basis of phenotypic data and phylogenetic inference, strain NEAU-3TGS17T was classified as representing a novel species in the genus Psychrobacillus , for which the name Psychrobacillus lasiicapitis sp. nov. is proposed. The type strain is NEAU-3TGS17T (=DSM 100484T=CGMCC 1.15308T).
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Rejection of reclassification of Lactobacillus kimchii and Lactobacillus bobalius as later subjective synonyms of Lactobacillus paralimentarius using comparative genomics
More LessLactobacillus bobalius , Lactobacillus kimchii and Lactobacillus paralimentarius belong to the genus Lactobacillus and show close phylogenetic relationships. In a previous study, L. bobalius and L. kimchii were proposed to be reclassified as later heterotypic synonyms of L. paralimentarius using high 16S rRNA gene sequence similarities (≥99.5 %) and DNA–DNA hybridization values (≥82 %). We determined high quality whole genome assemblies of the type strains of L. bobalius and L. kimchii , which were then compared with that of L. paralimentarius . Average nucleotide identity values among three genomes ranged from 91.4 to 92.3 % which are clearly below 95~96 %, the generally recognized cutoff value for bacterial species boundaries. On the basis of comparative genomic evidence, L. bobalius , L. kimchii , and L. paralimentarius should stand as separate species in the genus Lactobacillus . We therefore suggest rejecting the previous proposal to combine these three species into a single species.
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Salirhabdus salicampi sp. nov., a halotolerant bacterium isolated from a saltern
More LessA Gram-stain-positive, halotolerant bacterium, designated strain BH128T, was isolated from soil of a saltern located at Bigeum Island in south-west Korea. Cells were aerobic, motile, spore-forming rods and grew at 15–53 °C (optimum, 35 °C), at pH 5.5–9.0 (optimum, pH 7.0) and at salinities of 0–16 % (w/v) NaCl (optimum, 8 % NaCl). The predominant isoprenoid quinone was menaquinone-7 (MK-7), and the cell-wall peptidoglycan type was A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and four unknown phospholipids. The DNA G+C content was 36.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH128T belonged to the genus Salirhabdus and showed highest similarity to Salirhabdus euzebyi CVS-14T (95.8 %). On the basis of the phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH128T is considered to represent a novel species of the genus Salirhabdus , for which the name Salirhabdus salicampi sp. nov. is proposed. The type strain is BH128T (=KACC 18690T=NBRC 111874T).
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Rhabdanaerobium thermarum gen. nov., sp. nov., a novel anaerobic bacterium isolated from a hot spring
A novel Gram-stain-positive, obligately anaerobic and thermophilic bacterium, strain SYSU GA15001T, was isolated from a sample collected from a hot spring located in Tengchong, China. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SYSU GA15001T belonged to the family Eubacteriaceae , and was closely related to ‘ Irregularibacter muris ’ 2PG-426-CC4.2T (93.6 % similarity), Garciella nitratireducens DSM 15102T (93.2 %) and Alkalibacter saccharofermentans Z-78920T (89.7 %). Cells were rods and motile by means of a subpolar flagellum. Spores were formed in old cultures. Optimum growth was observed at 45 °C, at pH 7 and in the presence of 1.5 % (w/v) NaCl. The strain utilized d-arabinose, dulcitol, d-galactose, glucose, lactose, maltose, d-mannose, raffinose, l-rhamnose, d-sorbitol, xylitol, l-alanine, l-arginine, glycine, l-isoleucine, l-leucine, dl-methionine, NH4Cl, l-proline and l-threonine as sole carbon/nitrogen sources. Additionally, complex substrates including beef extract, peptone, starch and yeast extract can also be used as sole energy sources. The major fatty acid methyl esters were C14 : 0, anteiso-C15 : 0 and C18 : 0. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified polar lipid, three unidentified phospholipids and an unidentified glycolipid. The DNA G+C content was determined to be 35.6 mol%. Strain SYSU GA15001T was physiologically and phylogenetically distinguishable from its closely related genera, and merits assignment as representing the type species of a new genus Rhabdanaerobium, with the name Rhabdanaerobium thermarum gen. nov., sp. nov. The type strain of the type species is SYSU GA15001T (=CGMCC 1.5219T=KCTC 15623T).
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Caecibacterium sporoformans gen. nov., sp. nov., an anaerobic, butyrate-producing, spore-forming bacterium isolated from chicken caecum
Strains of a Gram-stain-negative, rod-shaped and immotile bacterium were isolated from broiler chicken caecal content. The isolates required strict anaerobic conditions for growth, formed spores, were catalase-positive and oxidase-negative. They produced butyrate as the major metabolic end product in reinforced clostridial medium broth. The genomic DNA G+C content of the isolated strains was 32.5–34.6 mol%. The major cellular fatty acids were C16 : 0 FAME, C14 : 0 FAME, C19 : 0CYC 9,10DMA and C16 : 0DMA. The fatty acid composition of the cell wall showed no similarity to any strain in the midi database. 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbours were Anaerostipes hadrus and Clostridium populeti (92 % sequence similarity) within Clostridium cluster XIVa of the phylum Firmicutes . Therefore, a novel genus is proposed, with the name Caecibacterium sporoformans gen. nov., sp. nov. The type strain of Caecibacterium sporoformans is LMG 27730T=DSM 26959T.
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Bacillus ciccensis sp. nov., isolated from maize (Zea mays L.) seeds
Two Gram-stain-positive bacterial strains, designated as 5L6T and 6L6, isolated from seeds of hybrid maize (Zea mays L., Jingke 968) were investigated using a polyphasic taxonomic approach. The cells were aerobic, motile, endospore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were recognized as a species of the genus Bacillus , to which the five closest neighbours are Bacillus solani FJAT-18043T (99.8 % similarity), Bacillus horneckiae DSM 23495T (97.7 %), Bacillus eiseniae A1-2T (97.4 %), Bacillus kochii WCC 4582T (97.1 %) and Bacillus purgationiresistens DS22T (97.0 %). The DNA G+C content of strain 5L6T was 37.4 mol%. Its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was MK-7 and the major fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C14 : 0, anteiso-C17 : 0 and C16 : 1 ω7c alcohol. The cell-wall peptidoglycan contained ornithine, serine, aspartic acid, glutamic acid and alanine while diaminopimelic acid could not be detected. Strains 5L6T and 6L6 were clearly distinguished from the type strains of related validly named species using phylogenetic analysis, DNA–DNA hybridization, fatty acid analysis, peptidoglycan analysis and comparison of a range of physiological and biochemical characteristics. The genotypic and phenotypic data show that strains 5L6T and 6L6 represent a novel species of the genus Bacillus, for which the name Bacillus ciccensis sp. nov. is proposed. The type strain is 5L6T (=KCTC 33663T=CICC 23855T=DSM 104513T).
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Paenibacillus albidus sp. nov., isolated from grassland soil
More LessA novel bacterial strain, designed Q4-3T, was isolated from a soil sample obtained from Qilian grassland, Qinghai, China. Phylogenetic, phenotypic, chemotaxonomic and molecular analyses were performed on the new isolate. Cells were Gram-stain-positive, facultatively anaerobic, spore-forming, motile rods with peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences placed strain Q4-3T in the genus Paenibacillus, and its closest relatives were Paenibacillus odorifer JCM 21743T, Paenibacillus typhae DSM 25190T, Paenibacillus borealis DSM 13188T and Paenibacillus etheri DSM 29760T with 16S rRNA gene sequence similarities of 98.12, 97.89, 97.63 and 97.6 %, respectively. The isolate grew at 4-37 °C (optimum 28-30 °C), at pH 6.0-10.0 (optimum pH 7.5) and with 0-3 %(w/v) NaCl (optimum 1 %). The DNA of strain Q4-3T was determined to be 48.6 mol%. The predominant menaquinone was MK-7 and the diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid. Anteiso-C15 : 0 (55.5 %), iso-C16 : 0 (14.5 %) and C16 : 0 (13.3 %) were the major fatty acids. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified lipid. Based on these results, strain Q4-3T is considered to represent a novel of the genus Paenibacillus, for which the name Paenibacillus albidus nov. is proposed. The type strain is Q4-3T (=CGMCC 1.16134T=KCTC 33911T).
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Anoxynatronum buryatiense sp. nov., an anaerobic alkaliphilic bacterium from a low mineralization soda lake in Buryatia, Russia
An anaerobic alkaliphilic, proteolytic bacterium, strain Su22T, was isolated from the bottom sediment of the alkaline low mineralization lake Sulphatnoe (Selenginsky district, Buryatia, Russia). A comparative analysis of the 16S rRNA gene sequence revealed that this bacterium was closely related to Anoxynatronum sibiricum Z-7981T with a similarity of 98.1 %. Strain Su22T differed from A. sibiricum Z-7981T in its inability to use carbohydrates, peptone and amino acids as carbon sources. Strain Su22T grew over a temperature range of 20–40 °C with an optimum at 30 °C and within the pH range 7.4–11.0 with an optimum at pH 9.6. Sodium cations stimulated the growth of the strain considerably with an optimal concentration at 0.76–1.09 M. The whole-cell fatty acid profile included C16 : 1ω7c, C16 : 0 and C16 : 0 ALDE. The G+C content was 46.1 mol%. Based on the DNA–DNA hybridization level (53.2 %) and phenotypical differences between strains Su22T and Z-7981T, the new isolate is thus considered to represent a novel species, for which the name Anoxynatronum buryatiense sp. nov. is proposed. The type strain is Su22Т (=VKM B-2510T=CECT 8731T).
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Cohnella algarum sp. nov., isolated from a freshwater green alga Paulinella chromatophora
Yunho Lee and Che Ok JeonA Gram-stain-positive, facultatively aerobic and endospore-forming bacterium, designated strain Pch-40T, was isolated from a freshwater green alga, Paulinella chromatophora. Cells were motile rods with a monotrichous polar flagellum showing catalase- and oxidase-positive reactions. Strain Pch-40T grew at 20–50 °C (optimum, 37–40 °C), at pH 5.0–11.0 (optimum, pH 7.0) and in the presence of 0–4.0 % (w/v) NaCl (optimum, 0 %). Menaquinone-7 was detected as the sole isoprenoid quinone. The genomic DNA G+C content of strain Pch-40T was 55.6 mol%. The major cellular fatty acids of strain Pch-40T were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Pch-40T clearly belonged to the genus Cohnella of the family Paenibacillaceae . Strain Pch-40T was most closely related to Cohnella rhizosphaerae CSE-5610T with a 96.1 % 16S rRNA gene sequence similarity. The phenotypic and chemotaxonomic features and the phylogenetic inference clearly suggested that strain Pch-40T represents a novel species of the genus Cohnella , for which the name Cohnella algarum sp. nov. is proposed. The type strain is strain Pch-40T (=KACC 19279T=JCM 32033T).
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Aeribacillus composti sp. nov., a thermophilic bacillus isolated from olive mill pomace compost
A Gram-stain-positive, aerobic, endospore-forming, thermophilic bacterium, strain N.8T, was isolated from the curing step of an olive mill pomace compost sample, collected at the Composting Experimental Centre (CESCO, Salerno, Italy). Strain N.8T, based on 16S rRNA gene sequence similarities, was most closely related to Aeribacillus pallidus strain H12T (=DSM 3670T) (99.8 % similarity value) with a 25 % DNA–DNA relatedness value. Cells were rod-shaped, non-motile and grew optimally at 60 °C and pH 9.0, forming cream colonies. Strain N.8 was able to grow on medium containing up to 9.0 % (w/v) NaCl with an optimum at 6.0 % (w/v) NaCl. The cellular membrane contained MK-7, and C16 : 0 (48.4 %), iso-C17 : 0 (19.4 %) and anteiso-C17 : 0 (14.6 %) were the major cellular fatty acids. The DNA G+C content was 40.5 mol%. Based on phenotypic characteristics, 16S rRNA gene sequences, DNA–DNA hybridization values and chemotaxonomic characteristics, strain N.8T represents a novel species of the genus Aeribacillus , for which the name Aeribacillus composti sp. nov. is proposed. The type strain is N.8T (=KCTC 33824T=JCM 31580T).
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