1887

Abstract

SUMMARY

Several substances in urine were found to inhibit the radioimmunoassay of added gonococcal antigens. The supernatants of two-thirds of urine samples from male patients with either gonorrhoea or non-specific urethritis (NSU) were inhibitory. The inhibition caused by many, but not all, samples was reduced or completely abolished by the addition of soybean trypsin inhibitor (STI); STI-sensitive inhibition is thought to be due to proteolytic enzymes, probably from pus cells. Their inhibitory effect was shown to be due to their action on gonoccocal antigens and not on antibodies in the assay system. Some supernatants contained other inhibitors unaffected by STI; some of these were dialysable and others were not.

Sediments from the urine of patients with NSU or gonorrhoea were often strongly inhibitory, but treatment with STI annulled all but very slight inhibition. STI-treated sediments could, therefore, be used in an assay designed to detect gonococcal antigens.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-12-2-177
1979-05-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/12/2/medmicro-12-2-177.html?itemId=/content/journal/jmm/10.1099/00222615-12-2-177&mimeType=html&fmt=ahah

References

  1. Dewald B., Rindler-Ludwig R., Bretz U., Baggiolini M. 1975; Subcellular localization and heterogeneity of neutral proteases in neutrophilic polymorphonuclear leukocytes. J. exp. Med 141:709
    [Google Scholar]
  2. Girard J., Greenwood F. C. 1968; The absence of intact growth hormone in urine as judged by radioimmunoassay. J. Endocrin 40:493
    [Google Scholar]
  3. Johnston K. H., Holmes K. K., Gotschlich E. C. 1976; The serological classification of Neisseria gonorrhoeae I. Isolation of the outer membrane complex responsible for serotypic specificity. J. exp. Med 143:741
    [Google Scholar]
  4. Maxfield M. 1966; Urinary glycoprotein. In Glycoproteins edited by Gottschalk A. London: pp 446–461
    [Google Scholar]
  5. Starkey P. M., Barrett A. J. 1976a; Neutral proteinases of human spleen. Purification and criteria for homogeneity of elastase and cathepsin G. Biochem. J 155:255
    [Google Scholar]
  6. Starkey P. M., Barrett A. J. 1976b; Human lysosomal elastase. Catalytic and immunological properties. Biochem. J 155:265
    [Google Scholar]
  7. Starkey P. M., Barrett A. J. 1976c; Human cathepsin G. Catalytic and immunological properties. Biochem. J 155:273
    [Google Scholar]
  8. Thorne K. J. I., Oliver R. C., Barrett A. J. 1976; Lysis and killing of bacteria by lysosomal proteinases. Infect. Immun 14:555
    [Google Scholar]
  9. Thornley M. J., Wilson D. V., Demarco de Hormaeche R., Oates J. K., Coombs R. R. A. 1979; Detection of gonococcal antigens in urine by radioimmunoassay. J. med. Microbiol 12:161
    [Google Scholar]
  10. Wide L. 1969; Radioimmunoassays employing immunosorbents. Acta Endocr., Copenhagen 63: (suppl. 142) 207
    [Google Scholar]
  11. Wilson D. V., Thornley M. J., Coombs R. R. A. 1977; A solid-phase assay with radioactively labelled antibody for the detection of Brucella abortus. J. Med. Microbiol 10:281
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-12-2-177
Loading
/content/journal/jmm/10.1099/00222615-12-2-177
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error