Quantitative cell-adhesion assay for Clostridium difficile cytotoxin

References

1. Adler E M, Fluk L J, Mullin J M, Kleinzeller A. 1982; Anomalous patterns in cultured cell monolayers. Science 217:851–853
   [Google Scholar]
2. Aronsson B, Granström M, Möllby R, Nord C E. 1983; Enzyme-linked immunosorbent assay (ELISA) for antibodies to Clostridium difficile toxins in patients with pseudo-membranous colitis and antibiotic-associated diarrhoea. Journal of Immunological Methods 60:341–350
   [Google Scholar]
3. Barer M, Lyon H O, Drasar B S. 1986; Quantitation of dye-binding to cell monolayers in a microtitre system. Histo-chemistry 18:122–128
   [Google Scholar]
4. Bartlett J G, Onderdonk A B, Cisneros R L, Kasper D L. 1977; Clindamycin-associated colitis due to a toxin-producing species of Clostridium in hamsters. Journal of Infectious Diseases 136:701–705
   [Google Scholar]
5. Bartlett J G, Chang T W, Gurwith M, Gorbach S L, Onderdonk A B. 1978; Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia. New England Journal of Medicine 298:531–534
   [Google Scholar]
6. Baumgarten H. 1985; A simple microplate assay for the determination of cellular protein. Journal of Immunological Methods 82:25–37
   [Google Scholar]
7. Borriello S P, Honour P. 1981; Simplified procedure for the routine isolation of Clostridium difficile from faeces. Journal of Clinical Pathology 34:1124–1126
   [Google Scholar]
8. Bradford M M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Analytical Biochemistry 72:248–254
   [Google Scholar]
9. Chang T-W, Gorbach S L, Bartlett J B. 1978; Neutralization of Clostridium difficile toxin by Clostridium sordellii antitoxins. Infection and Immunity 22:418–422
   [Google Scholar]
10. Connolly D T, Knight M B, Harakas N K, Wittwer A J, Feder J. 1986; Determination of the number of endothelial cells in culture using an acid phosphatase assay. Analytical Biochemistry 152:136–140
    [Google Scholar]
11. Curtis A S G, Forrester J V, Mclnnes C, Lawrie F. 1983; Adhesion of cells to polystyrene surfaces. Journal of Cell Biology 97:1500–1506
    [Google Scholar]
12. Fazekas de St Groth S, Webster R G, Datyner A. 1963; Two new staining procedures for quantitative estimation of proteins on electrophoretic strips. Biochimica et Biophysica Acta 71:377–391
    [Google Scholar]
13. Florin I, Thelestam M. 1981; Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infection and Immunity 33:67–74
    [Google Scholar]
14. George R H. 1978; Identification of Clostridium difficile as a cause of pseudomembranous colitis. British Medical Journal 1:695
    [Google Scholar]
15. George W L, Rolfe R D, Finegold S M. 1982; Clostridium difficile and its cytotoxin in feces of patients with antimicrobial agent-associated diarrohea and miscellaneous conditions. Journal of Clinical Microbiology 15:1049–1053
    [Google Scholar]
16. Knox P. 1984; Kinetics of cell spreading in the presence of different concentrations of serum or fibronectin-depleted serum. Journal of Cell Science 71:51–59
    [Google Scholar]
17. Laemmli U K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 111:680–685
    [Google Scholar]
18. Laughton C. 1984; Quantification of attached cells in microtiter plates based on Coomassie Brilliant blue G-250 staining of total cellular protein. Analytical Biochemistry 140:417–423
    [Google Scholar]
19. Libby J M, Jortner B S, Wilkins T D. 1982; Effects of the two toxins of Clostridium difficile in antibiotic-associated cecitis in hamsters. Infection and Immunity 36:822–829
    [Google Scholar]
20. McClane B A, McDonel J L. 1979; The effects of Clostridium perfringens enterotoxin on morphology, viability, and macromolecular synthesis in Vero cells. Journal of Cellular Physiology 99:191–200
    [Google Scholar]
21. Montesano R, Roth J, Robert A, Orci L. 1982; Non-coated membrane invaginations are involved in binding and internalization of cholera and tetanus toxins. Nature 296:651–653
    [Google Scholar]
22. Nath K, Srere P A. 1977; Effects of temperature, metabolic and cystoskeletal inhibitors on the rate of BHK cell adhesion to polystyrene. Journal of Cellular Physiology 92:33–42
    [Google Scholar]
23. Patterson M K. 1979; Measurement of growth and viability of cells in culture. Methods in Enzymology 58:141–152
    [Google Scholar]
24. Peterson L R, Holter J J, Shanholtzer C J, Garrett C R, Gerding D N. 1986; Detection of Clostridium difficile toxins A (enterotoxin) and B (cytotoxin) in clinical specimens. Evaluation of a latex agglutination test. American Journal of Clinical Pathology 86:208–211
    [Google Scholar]
25. Pothoulakis C. 1986; Purification and properties of Clostridium difficile cytotoxin B. Journal of Biological Chemistry 261:1316–1321
    [Google Scholar]
26. Rothman S W, Brown J E, Diecidue A, Foret D A. 1984; Differential cytotoxic effects of toxins A and B isolated from Clostridium difficile . Infection and Immunity 46:324–331
    [Google Scholar]
27. Shopsis C, Mackay G J. 1984; A semi-automated protein assay for cell cultures. Analytical Biochemistry 140:104–107
    [Google Scholar]
28. Taylor N S, Thorne G M, Bartlett J G. 1981; Comparison of two toxins produced by Clostridium difficile. Infection and Immunity 34:1036–1043
    [Google Scholar]
29. Thelestam M, Bronnegard M. 1980; Interaction of cytopathogenic toxin from Clostridium difficile with cells in tissue culture. Scandinavian Journal of Infectious Diseases2216–29
    [Google Scholar]
30. Vesely D L, Straub K D, Nolan C M, Rolfe R D, Finegold S M, Monson T P. 1981; Purified Clostridium difficile cytotoxin stimulates guanylate cyclase activity and inhibits adenylate cyclase activity. Infection and Immunity 33:285–291
    [Google Scholar]
31. Wedel N. 1983; Ultrastructural effects of Clostridium difficile toxin B on smooth muscle cells and fibroblasts. Experimental Cell Research 148:413–22
    [Google Scholar]
32. Yolken R H. 1981; Enzyme immunoassay for the detection of Clostridium difficile antigen. Journal of Infectious Diseases 144:378
    [Google Scholar]