1887

Abstract

Summary.

The bactericidal action of non-immune whole blood on was increased when the bacteria contained the R-plasmid RP1. This effect was due mainly to increased phagocytosis. Iron-depleted stationary-phase cells were more sensitive than carbon-depleted cells. The contribution of serum was usually negligible but was increased during a minor non-specific infection. Most plasmid-containing phenotypes were more sensitive than were those without plasmids but there were considerable differences between stationary and exponentially-growing cells. The R-plasmid-mediated increase in sensitivity to phagocytosis may be due in part to the presence of additional glycosylated proteins in the outer membrane.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-24-3-233
1987-11-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/jmm/24/3/medmicro-24-3-233.html?itemId=/content/journal/jmm/10.1099/00222615-24-3-233&mimeType=html&fmt=ahah

References

  1. Batteiger B, Newhall W J, Jones R B. 1982; The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. Journal of Immunological Methods 55:297–307
    [Google Scholar]
  2. Brown M R W, Williams P. 1985; The influence of environment on envelope properties affecting survival of bacteria in infections. Annual Review of Microbiology 39:527–556
    [Google Scholar]
  3. Diem K, Lentner C. 1970; Total leucocyte count and distribution at various ages. Documenta Geigy, 7. Ciba-Geigy; Basle:619
    [Google Scholar]
  4. Easmon C S F, Cole P J, Williams A J, Hastings M. 1980; The measurement of opsonic and phagocytic function by luminol-dependent chemiluminescence. Immunology 41:67–74
    [Google Scholar]
  5. Filip C, Fletcher A, Wilf J, Earhart C F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  6. Griffiths E. 1983; Availability of iron and survival of bacteria in infection.. Easmon C S F. Role of the envelope in the survival of bacteria in infection, Medical Microbiology 3 Academic Press; London:153–177
    [Google Scholar]
  7. Levin B R. 1980; Conditions for the existence of R-plasmids in bacterial populations. Mitsuhashi S. Antibiotic resistance : transposition and other mechanisms Springer Verlag; Berlin:197
    [Google Scholar]
  8. Lowbury E J L, Kidson A, Lilly H A, Ayliffe G A J, Jones R J. 1969; Sensitivity of Pseudomonas aeruginosa to antibiotics : emergence of strains highly resistant to carbenicillin. Lancet 2:448–452
    [Google Scholar]
  9. Lugtenberg B, Meijers J, Peters R, van der Hoek P, van Alphen L. 1975; Electrophoretic resolution of the “major outer membrane protein” of Escherichia coli K12 into four bands. FEBS Letters 58:254–258
    [Google Scholar]
  10. MacManus A T, Northam W J, Moody E E M. 1981; Suppression of Pseudomonas aeruginosa burn surface infection by plasmid RP1. Abstracts of the Annual Meeting American Society for Microbiology;17
    [Google Scholar]
  11. Onaolapo J A. 1986; Effect of R-plasmid RP1 on the properties of Proteus mirabilis . PhD thesis, Aston University
    [Google Scholar]
  12. Onaolapo J A, El-Haffar M I A, Townley D, Klemperer R M M. 1987; R-plasmid RP1 promotes adhesion of Gram-negative bacteria to medical prostheses and glass. Journal of Medical Microbiology 24:227–232
    [Google Scholar]
  13. Onaolapo J A, Klemperer R M M. 1986; Effect of R-plasmid on surface hydrophobicity of Proteus mirabilis . Journal of General Microbiology 132:3303–3307
    [Google Scholar]
  14. Shand G. H, Anwar H, Kadurugamuwa J, Brown M R W, Silverman S H, Melling J. 1985; In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions. Infection and Immunity 48:35–39
    [Google Scholar]
  15. Smith H. 1984; The biochemical challenge of microbial pathogenicity. Journal of Applied Bacteriology 57:395–404
    [Google Scholar]
  16. Stahl P D, Rodman J S, Miller M J, Schlesinger P H. 1978; Evidence for receptor-mediated binding of glycoproteins, glycoconjugates and lysosomal glycosidases by alveolar macrophages. Proceedings of the National Academy of Sciences of the USA 75:1399–1403
    [Google Scholar]
  17. Stendahl O. 1983; The physicochemical basis of surface interaction between bacteria and phagocytic cells. Easmon C S F. Role of the envelope in the survival of bacteria in infection. Medical Microbiology 3 Academic Press; London:137–152
    [Google Scholar]
  18. Taylor P W. 1983; Bactericidal and bacteriolytic activity of serum against Gram-negative bacteria. Microbiological Reviews 47:46–83
    [Google Scholar]
  19. Towbin H, Staehelin T, Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications. Proceedings of the National Academy of Sciences of the USA 76:4350–4354
    [Google Scholar]
  20. Tsang V C W, Peralta J M, Simons A R. 1983; Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods in Enzymology 92:377–391
    [Google Scholar]
  21. Williams P, Lambert P A, Haigh C G, Brown M R W. 1986; The influence of the O and K antigens of Klebsiella aerogenes on surface hydrophobicity and susceptibility to phagocytosis and antimicrobial agents. Journal of Medical Microbiology 21:125–132
    [Google Scholar]
  22. Wretlind B, Becker K, Haas D. 1985; IneP-1 R-plasmidsdecrease the serum resistance and the virulence of Pseudomonas aeruginosa . Journal of General Microbiology 131:2701–2704
    [Google Scholar]
  23. Wright B L, Carrick L, Jollick J D. 1977; The effect of an R-factor on the virulence of Pseudomonas aeruginosa in the mouse. Abstracts of the Annual Meeting American Society for Microbiology;35
    [Google Scholar]
  24. Xiu J H, Magnusson K E, Stendahl O, Edebo L. 1983; Physiochemical surface properties and phagocytosis by polymorphonuclear leucocytes of different serogroups of Salmonella . Journal of General Microbiology 129:3075–3084
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-24-3-233
Loading
/content/journal/jmm/10.1099/00222615-24-3-233
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error