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Volume 38, Issue 1

Survival of group A streptococci in dried human blood Free

Abstract

Summary

The resurgence of streptococcal infections in the USA and Europe and their high incidence in other parts of the world prompted an examination of the survival and maintenance of virulence of group A streptococci. Human blood containing group A streptococci was placed on small pieces of sterile paper towelling and allowed to dry at room temperature. At periods of 2, 8, 15 and 20 weeks later, the paper with the dried blood was placed in Todd-Hewitt broth and incubated at 37°C overnight. All the samples tested at 2 weeks grew in broth, and with only one exception, grew in fresh human blood provided by five donors. At 8 weeks only two of the 10 strains failed to grow in broth; seven of the eight viable cultures also grew in blood. At 15 and 20 weeks after drying the eight cultures were still viable. Since seven were able to grow in fresh blood as well as in broth it is assumed that their virulence factor(s) had been retained.

  • Received:
  • Accepted:
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  • Published Online:
© Society for General Microbiology, 1993
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1993-01-01
2024-05-04
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References

  1. Agarwal BL. Rheumatic heart disease unabated in developing countries. Lancet 1981; 1:910–911
     [Google Scholar]
  2. Asay E, Giles R, Veasy LG, Hill HR, Ware J, Nicholes HR. Acute rheumatic fever-Utah. MMWR 1987; 36:108–110115
     [Google Scholar]
  3. Congeni B, Rizzo C, Congeni J, Sreenivasan VV. Outbreak of acute rheumatic fever in northeast Ohio. J Pediatr 1987; 111:176–179
     [Google Scholar]
  4. Hosier DM, Craenan JM, Teske DW, Wheller JJ. Resurgence of acute rheumatic fever. Am J Dis Child 1987; 141:730–733
     [Google Scholar]
  5. Veasy LG, Weidmeier SE, Orsmond GS. et al. Resurgence of acute rheumatic fever in the intermountain area of the United States. N Engl J Med 1987; 316:421–427
     [Google Scholar]
  6. Wald ER, Dashefsky B, Feidt C, Chiponis D, Byers C. Acute rheumatic fever in western Pennsylvania and the tristate area. Pediatrics 1987; 80:371–374
     [Google Scholar]
  7. Papadimos T, Escamilla J, Garst P. , Centers for Disease Control Acute rheumatic fever at a Navy Training Center—San Diego, California. MMWR 1988; 37:101–104
     [Google Scholar]
  8. Sampson GL, Williams RG, House MD. , Centers for Disease Control Acute Rheumatic fever among Army trainees—Fort Leonard Wood, Missouri, 1977-1988. MMWR 1988; 37:519–522
     [Google Scholar]
  9. Land MA, Bisno AL. Acute Rheumatic fever: a vanishing disease in suburbia. JAMA 1983; 249:895–898
     [Google Scholar]
  10. Cone LA, Woodard DR, Schlievert PM, Tomory GS. Clinical and bactériologie observations of a toxic shock-like syndrome due to Streptococcus pyogenes. N Engl J Med 1987; 317:146–149
     [Google Scholar]
  11. Bartter T, Dascal A, Carroll K, Curley FJ. Toxic strep syndrome: a manifestation of group A streptococcal infection. Arch Intern Med 1988; 148:1421–1424
     [Google Scholar]
  12. Stevens DL, Tanner MH, Winship J. et al. Severe group A streptococcal infections associated with a toxic shock-like syndrome and scarlet fever toxin A. N Engl J Med 1989; 321:1–7
     [Google Scholar]
  13. Voeck C, Armstrong N, Trail F. et al.Centers for Disease Control Group A beta-hemolytic streptococcal bacteremia—Colorado, 1988. MMWR 1990; 39:3–6
     [Google Scholar]
  14. Wheeler MC, Roe MH, Kapan EL, Schlievert PM, Todd JK. Outbreak of group A streptococcus septicemia in children. Clinical, epidemiologic, and microbiologie correlates. JAMA 1991; 266:533–537
     [Google Scholar]
  15. Hartsough AB, Henry TE, Parks BO. Sudden death due to streptococcal infection. J Forensic Sci 1991; 36:1485–1491
     [Google Scholar]
  16. Rammelkamp CH, Denny FW, Wannamaker LW. In: Thomas L. (ed) Rheumatic fever (Studies on the epidemiology of rheumatic fever in the armed services) Minneapolis: University of Minnesota Press; 195272–89
     [Google Scholar]
  17. Todd EW, Lancefield RC. Variants of hemolytic streptococci; their relationship to type-specific substance, virulence, and toxins. J Exp Med 1928; 48:751–767
     [Google Scholar]
  18. Lancefield RC. Current knowledge of type-specific M antigens of group A streptococci. J Immunol 1962; 89:307–313
     [Google Scholar]
  19. van de Rijn I, Fischetti VA. Immunochemical analysis of intact M protein secreted from cell wall-less streptococci. Infect Immun 1981; 32:86–91
     [Google Scholar]
  20. Manjula BN. Molecular aspects of the phagocytosis resistance of group A streptococci. Eur J Epidemiol 1988; 4:289–300
     [Google Scholar]
  21. Fischetti VA. Streptococcal M protein: molecular design and biological behaviour. Clin Microbiol Rev 1989; 2:285–314
     [Google Scholar]
  22. Wilson AT. The relative importance of the capsule and the M-antigen in determining colony form of group A streptococci. J Exp Med 1959; 109:257–270
     [Google Scholar]
  23. Facklam RR, Carey RB. Streptococci and aerococci. In: Lennetti EH. (ed) Manual of clinical microbiology, 4th edn. Washington, D.C: American Society for Microbiology; 1985156–157
     [Google Scholar]
  24. Wessels MR, Moses AE, Goldberg JB, DiCesare TJ. Hyaluronic acid capsule is a virulence factor for mucoid group A streptococci. Proc Natl Acad Sci USA 1991; 88:8317–8321
     [Google Scholar]
  25. del Rio Gomez I, Bratthall D. Sample, count, identify and store the mutans streptococci. Scand J Dent Res 1990; 98:106–111
     [Google Scholar]
  26. Maxted WR. The indirect bactericidal test as a means of identifying antibody to the M antigen of Streptococcus pyogenes. Br J Exp Pathol 1956; 37:415–422
     [Google Scholar]
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Survival of group A streptococci in dried human blood
J. Med. Microbiol. 38, 61 (1993); https://doi.org/10.1099/00222615-38-1-61
/content/journal/jmm/10.1099/00222615-38-1-61
/content/journal/jmm/10.1099/00222615-38-1-61
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