1887

Abstract

The importance of as a pathogen in premature neonates and patients with a profound defect in humoral immunity has, over the last few years, become well recognised. is unique amongst the Mycoplasmataceae for its use of urea metabolism as an essential source of energy. The urease enzyme responsible for this is, therefore, of prime importance and any variability in expression of this enzyme may play a role in virulence of the organism. is divided into 14 serovars comprising two biovars-the parvo-biovar and T960-biovar. In this study monoclonal antibodies (MAbs) were produced against the urease enzyme. Two distinct epitopes of the 72-kDa α-subunit were recognised by three different MAbs. Under denaturing conditions both epitopes were shown to be specific for the parvobiovar.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-45-5-366
1996-11-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/jmm/45/5/medmicro-45-5-366.html?itemId=/content/journal/jmm/10.1099/00222615-45-5-366&mimeType=html&fmt=ahah

References

  1. Cassell G. H., Davis R. O., Waites K. B. Isolation of Mycoplasma hominis and Ureaplasma urealyticum from amniotic fluid at 16-20 weeks of gestation: potential effect on outcome of pregnancy. Sex Transm Dis 1983; 10: Suppl 294–302
    [Google Scholar]
  2. Taylor-Robinson D., Furr P. M., Webster A. D. B. Ureaplasma urealyticum in the immunocompromised host. Pediatr Infect Dis 1986; 5: (6 Suppl) S236–S238
    [Google Scholar]
  3. Waites K. B., Crouse D. T., Philips J. B., Canupp K. C., Ramsey C., Cassell G. H. Ureaplasmal pneumonia and sepsis associated with persistent pulmonary hypertension of the newborn. Pediatrics 1989; 83:79–85
    [Google Scholar]
  4. Cassell G. H., Waites K. B., Watson H. L., Crouse D. T., Harasawa R. Ureaplasma urealyticum intrauterine infection: role in prematurity and disease in newborns. Clin Microbiol Rev 1993; 6:69–87
    [Google Scholar]
  5. Stray-Pederson B., Eng J., Reikvam T.M. Uterine T-mycoplasma colonization in reproductive failure. Am J Obstet Gynecol 1978; 130:307–311
    [Google Scholar]
  6. Zheng X., Watson H. L., Waites K. B., Cassell G. H. Serotype diversity and antigen variation among invasive isolates of Ureaplasma urealyticum from neonates. Infect Immun 1992; 60:3472–3474
    [Google Scholar]
  7. Sterne G. W., Robertson J. A. Problems associated with serotyping strains of Ureaplasma urealyticum. Diagn Microbiol Infect Dis 1985; 3:311–320
    [Google Scholar]
  8. Christiansen C., Black F. T., Freundt E. A. Hybridization experiments with deoxyribonucleic acid from Ureaplasma urealyticum serovars I to VIII. Int J Syst Bacterial 1981; 31:259–262
    [Google Scholar]
  9. Razin S., Yogev D. Genetic relatedness among Ureaplasma urealvticum serotypes (serovars). Pediatr Infect Dis 1986; 5: (6 Suppl) S300–S304
    [Google Scholar]
  10. Robertson J. A., Chen M. H. Effects of manganese on the growth and morphology of Ureaplasma urealyticum. J Clin Microbiol 1984; 19:857–869
    [Google Scholar]
  11. Robertson J. A., Howard L. A., Zinner C. L., Stemke G. W. Relative homogeneity of the 16S rRNA genes within the T960- and the parvo-biovars of the ureaplasmas isolated from humans. 10M Lett 1994; 3:158
    [Google Scholar]
  12. Shepard M. C. Culture media for ureaplasmas. In Razin S., Tully J. G. (eds) Methods in mycoplasmology vol I New York: Academic Press; 1983137
    [Google Scholar]
  13. Davis J. W., Moses I. S., Ndubuka C., Ortiz R. Inorganic pyrophosphatase activity in cell-free extracts of Ureaplasma urealyticum. J Gen Microbiol 1987; 133:1453–1459
    [Google Scholar]
  14. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annal Biochem 1976; 72:248–254
    [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  16. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350–4353
    [Google Scholar]
  17. Razin S. Cell lysis and isolation of membranes. In Razin S., Tully J. G. (eds) Methods in mycoplasmology vol I New York: Academic Press; 1983225
    [Google Scholar]
  18. Edman P., Begg G. A protein sequenator. Eur J Biochem 1967; 1:80–91
    [Google Scholar]
  19. Blanchard A. Ureaplasma urealyticum urease genes; use of a UGA tryptophan codon. Mol Microbiol 1990; 4:669–676
    [Google Scholar]
  20. Romano N., La Licata R., Scarlata G. Immunological analysis of plasma membranes of a T-strain mycoplasma (Ureaplasma urealyticum). Infect Immun 1977; 16:734–737
    [Google Scholar]
  21. Swenson C. E., Van Hamont J., Dunbar B. S. Specific protein differences among strains of Ureaplasma urealyticum as determined by two-dimensional gel electrophoresis and a sensitive silver stain. Int J Syst Bacteriol 1983; 33:417–421
    [Google Scholar]
  22. Watson H. L., Blalock D. K., Cassell G. H. Variable antigens of Ureaplasma urealyticum containing both serovar-specific and serovar-cross-reactive epitopes. Infect Immun 1990; 58:3679–3688
    [Google Scholar]
  23. Thirkell D., Myles A. D., Russell W. C. Serotype 8- and serocluster-specific surface-expressed antigens of Ureaplasma urealyticum. Infect Immun 1989; 57:1697–1701
    [Google Scholar]
  24. Thirkell D., Myles A. D., Precious B. L. The urease of Ureaplasma urealyticum. J Gen Microbiol 1989; 135:315–323
    [Google Scholar]
  25. Thirkell D., Myles A. D., Taylor-Robinson D. A comparison of four major antigens in five human and several animal strains of ureaplasmas. J Med Microbiol 1990; 32:163–168
    [Google Scholar]
  26. Neyrolles O., Behbahani N., Ferris S., Montagnier L., Blanchard A. Characterisation and comparison of the urease operons in the two biovars of Ureaplasma urealyticum. IOM Lett 1994; 3:600
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-45-5-366
Loading
/content/journal/jmm/10.1099/00222615-45-5-366
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error