1887

Journal of Medical Microbiology logo

Volume 5, Issue 4

Examination By Electron Microscopy Of The Interaction Between Peritoneal Phagocytes And Free

Abstract

SUMMARY

The host-parasite interactions between (i) macrophagesand polymorphonuclear neutrophil leucocytes (PMN) of normal and immune mice, rabbits and guinea-pigs, and (ii) thefacultative intracellular parasite, , were examined with the electron-microscope. Normal mouse cells were incapable of exerting a significant bactericidal effect and quickly succumbed to the necrotising action of the organism. Macrophages from immune mice were individually no more effective in killing , but the organisms disappeared from the peritoneal cavities more rapidly in immune than in normal mice. Bacterial clearance appeared to be associated with the reingestion by scavenging macrophages of viable within degenerating phagocytes. Suspending cells in hyperimmune sheep serum rendered them susceptible to bacteriolysis by normal or immune mouse cells. Rabbit PMN exerted an extremely rapid destructive action on the organism; rabbit macrophages and guinea-pig PMN and macrophages also destroyed but less effectively. In studies with all three animal species, viable organisms were found within phagolysosomes which were characterised by a relatively narrow peribacillary space and crenate limiting membrane. An electron-lucent zone always surrounded viable bacteria, and this was assumed to indicate the intact state of the surface lipid layer which was of similar density to the contiguous host enzymic material. Bacteria undergoing degeneration were often found within vacuoles with a wide peribacillary space and smooth limiting membrane; the surface lipid zone was wholly or partly obliterated in these organisms. It appears that surface lipid may function as a barrier against the degradative enzymes of mouse phagocytes.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1972
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-5-4-483
1972-11-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jmm/5/4/medmicro-5-4-483.html?itemId=/content/journal/jmm/10.1099/00222615-5-4-483&mimeType=html&fmt=ahah

References

  1. Allen Jennifer M., Brieger E. M., Rees R. J. W. 1965; Electron microscopy of the host-cell parasite relation in murine leprosy. J. Path. Bad 89:301
     [Google Scholar]
  2. Bloch H. 1950; Studies on the virulence of tubercle bacilli. Isolation and biological properties of a constituent of virulent organisms. J. Exp. Med 91:197
     [Google Scholar]
  3. Brewer D. B. 1963; Electron microscopy of phagocytosis of staphylococci. J. Path. Bad 86:299
     [Google Scholar]
  4. Brown Carolyn A., Draper P. 1970; An electron-microscope study of rat fibroblasts infected with Mycobaderium lepraemurium. J. Path 102:21
     [Google Scholar]
  5. Brown Carolyn A., Draper P., Hart P. D. 1969; Mycobacteria and lysosomes: a paradox. Nature, Lond 221:658
     [Google Scholar]
  6. Carne H. R., Wickham Nancy, Kater Joan C. 1956; A toxic lipid from the surface of Corynebaderium ovis. Nature, Lond 178:701
     [Google Scholar]
  7. Chapman G. B., Hanks J. H., Wallace J. H. 1959; An electron microscope study of the disposition and fine structure of Mycobaderium lepraemurium in mouse spleen. J. Bad 77:205
     [Google Scholar]
  8. Christiansen R. G., Marshall J. M. 1965; A study of phagocytosis in the ameba Chaoschaos. J. Cell Biol 25:443
     [Google Scholar]
  9. Dickinson C. G., Bull L. B. 1931; Studies on infection by and resistance to the Preisz-Nocard bacillus. II. Susceptibility of the guinea-pig, and the distribution of lesions after cutaneous and subcutaneous inoculation and ingestion. Aust. J. Exp. Biol. Med. Sci 8:83
     [Google Scholar]
  10. Draper P., Rees R. J. W. 1970; Electron-transparent zone of mycobacteria may be a defence mechanism. Nature, Lond 228:860
     [Google Scholar]
  11. Dumont A., Sheldon H. 1965; Changes in the fine structure of macrophages in experimentally produced tuberculous granulomas in hamsters. Lab. Invest 14:2034
     [Google Scholar]
  12. Gomori G. 1950; An improved histochemical technic for acidphosphatase. Stain Technol 25:81
     [Google Scholar]
  13. Gordon G. B., Miller L. R., Bensch K. G. 1965; Studies on the intracellular digestive process in mammalian tissue culture cells. J. Cell Biol 25: no. 2, part 2 41
     [Google Scholar]
  14. Hard G. C. 1969a; Immunity to experimental infection with Corynebacterium ovis in the mouse peritoneal cavity. Res. Vet. Sci 10:547
     [Google Scholar]
  15. Hard G. C. 1969b; Electron microscopic examination of Corynebacterium ovis. J. Bad 97:1480
     [Google Scholar]
  16. Hard G. C. 1969C; Electron microscopic study of the differentiation of mouse peritoneal macrophages stimulated by Corynebacterium ovis infection. Lab. Invest 21:309
     [Google Scholar]
  17. Hard G. C. 1970; Some biochemical aspects of the immune macrophage. Br. J. Exp. Path 51:97
     [Google Scholar]
  18. Horn R. G., Koenig M. G., Goodman J. S., Collins R. D. 1969; Phagocytosis of Staphylococcus aureus by hepatic reticuloendothelial cells. An ultrastructural study. Lab. Invest 21:406
     [Google Scholar]
  19. Jolly R. D. 1965a; The pathogenesis of experimental Corynebacterium ovis infection in mice. N.Z. Vet.J 13:141
     [Google Scholar]
  20. Jolly R. D. 1965b; Experimental infection of convalescent mice with Corynebacterium ovis. N.Z. Vet. J 13:148
     [Google Scholar]
  21. Jolly R. D. 1966; Some observations on surface lipids of virulent and attenuated strains of Corynebacterium ovis. J. Appl. Bact 29:189
     [Google Scholar]
  22. Korn E. D., Weisman R. A. 1967; Phagocytosis of latex beads by Acanthamoeba. II. Electron microscopic study of the initial events. J. Cell Biol 34:219
     [Google Scholar]
  23. Leake Eva S., Myrvik Q. N. 1966; Digestive vacuole formation in alveolar macrophages after phagocytosis of Mycobacterium smegmatis in vivo. J. Reticuloendothel. Soc 3:83
     [Google Scholar]
  24. Lockwood W. R., Allison F. Jr 1964; Electron micrographic studies of phagocytic cells. II. Observations on the changes induced in the cytoplasmic contents of human granulocytes by the ingestion of rough pneumococcus. Br. J. Exp. Path 45:294
     [Google Scholar]
  25. North R. J., Mackaness G. B. 1963a; Electronmicroscopical observations on the peritoneal macrophages of normal mice and mice immunised with Listeria monocytogenes II.Structure of normal macrophages and the early cytoplasmic response to the presence of ingested bacteria. Br. J. Exp. Path 44:601
     [Google Scholar]
  26. North R. J., Mackaness G. B. 1963b; Electronmicroscopical observations on the peritoneal macrophages of normal mice and mice immunised with Listeria monocytogenes.II.Structureof macrophages from immune mice and early cytoplasmic response to the presence of ingested bacteria. Br. J. Exp. Path 44:608
     [Google Scholar]
  27. Trump B. F., Bulger R. E. 1966; New ultrastructural characteristics of cells fixed in a glutaraldehyde-osmium tetroxide mixture. Lab. Invest 15:368
     [Google Scholar]
  28. Yamamoto T., Nishiura M., Harada N., Imaeda T. 1958; Electron microscopy of Mycobacterium lepraemurium in ultra-thin sections of murineleprosy lesions. Int. J. Lepr 26:111
     [Google Scholar]
  29. Zucker-franklin Dorothy, Hirsch J. G. 1964; Electron microscope studies on the degranulation of rabbit peritoneal leukocytes during phagocytosis. J. Exp. Med 120:569
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-5-4-483
Loading
Examination By Electron Microscopy Of The Interaction Between Peritoneal Phagocytes And Corynebacterium Ovis
J. Med. Microbiol. 5, 483 (1972); https://doi.org/10.1099/00222615-5-4-483
/content/journal/jmm/10.1099/00222615-5-4-483
/content/journal/jmm/10.1099/00222615-5-4-483
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error