1887

Abstract

SUMMARY

A defined medium containing mineral salts, glucose and vitamins was developed in which large amounts of enterotoxin were produced by Escherichia coli strain P16 (serotype 09; K9) if the culture was well aerated during incubation for at least 48 hours and the pH was controlled to between 7.4 and 8.1.

After gel filtration of the supernates, the fractions with enterotoxic activity obtained from cultures in the defined medium contained considerably less ultra-violet-light absorbent material than did the corresponding fractions from cultures in “syncase” medium.

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/content/journal/jmm/10.1099/00222615-7-3-395
1974-08-01
2024-05-06
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References

  1. Bywater R. J. 1972; Dialysis and ultrafiltration of a heat-stable enterotoxin from Escherichia coli. J. med. Microbiol. 5:337
    [Google Scholar]
  2. Callahan L. T., Richardson S. H. 1973; Biochemistry of Vibrio cholerae virulence. III. Nutritional requirements for toxin production and the effects of pH on toxin elaboration in chemically defined media. Infect. Immun 7:567
    [Google Scholar]
  3. De S. N., Chatterjee D. N. 1953; An experimental study on the mechanism of action of Vibrio cholerae on the intestinal mucous membrane. J. Path. Bact 66:559
    [Google Scholar]
  4. Evans D. J., Chen L. C., Curlin G. T., Evans D. G. 1972; Stimulation of adenyl cyclase by Escherichia coli enterotoxin. Nature New Biol., Lond 236:137
    [Google Scholar]
  5. Finkelstein R. A., Norris H. T., Dutta N. K. 1964; Pathogenesis of experimental cholera in infant rabbits. 1. Observations on the intra-intestinal infection and experimental cholera produced with cell-free products. J. infect. Dis 114:203
    [Google Scholar]
  6. Kohler E. M. 1968; Enterotoxic activity of filtrates of Escherichia coli in young pigs. Am. J. vet. Res 29:2263
    [Google Scholar]
  7. Lariviére S. 1971; Physical and biological characterisation of Escherichia coli enterotoxin. Ph.D. Thesis University of Guelph; Canada:
    [Google Scholar]
  8. Moon H. W., Sorensen D. K., Sautter J. H. 1966; Escherichia coli infection of the ligated intestinal loop of the newborn pig. Am. J. vet. Res 27:1317
    [Google Scholar]
  9. Moon H. W., Whipp S. C., Baetz A. L. 1971; Comparative effects of enterotoxins from Escherichia coli and Vibrio cholerae on rabbit and swine small intestine. Lab. Invest 25:133
    [Google Scholar]
  10. Smith H. W., Gyles C. L. 1970; The effect of cell-free fluids prepared from cultures of human and animal enteropathogenic strains of Escherichia coli on ligated intestinal segments of rabbits and pigs. J. med. Microbiol 3:403
    [Google Scholar]
  11. Smith H. W., Halls S. 1967; Studies on Escherichia coli enterotoxin. J. Path. Bact 93:531
    [Google Scholar]
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