1887

Journal of Medical Microbiology logo

Volume 8, Issue 2

INDUCTION OF MUTATION IN BY ETHYLMETHANE SULPHONATE Free

Abstract

SUMMARY

strains were treated with ethylmethane sulphonate with the object of obtaining mutants that differed from the wild type in one of the properties that might contribute to virulence.

Mutants with isolated loss or deficient production of α-haemolysin, coagulase, leucocidin and staphylokinase were obtained. Changes in more than one property were often observed. The loss of δ-lysin production was always coupled with an appreciable reduction in P-V leucocidin production. With that exception, systematised combined loss of a restricted number of staphylococcal characteristics was not observed. Mutants that had lost all characteristics of were phage-resistant. Isolated loss of staphylokinase production and deficient P-V leucocidin production were found to be due to the independent loss of two prophages.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1975
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-8-2-265
1975-05-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/8/2/medmicro-8-2-265.html?itemId=/content/journal/jmm/10.1099/00222615-8-2-265&mimeType=html&fmt=ahah

References

  1. Agarwal D. S. 1967; Subcutaneous staphylococcal infection in mice. II. The inflammatory response to different strains of staphylococci and micrococci. Br. J. exp. Path 48:468
     [Google Scholar]
  2. Arbuthnott J. P. 1970; Staphylococcal α-toxin. In Microbial toxins vol III: edited by Montie T. C. Kadis S., Ajl S. J. New York and London: p 189
     [Google Scholar]
  3. Arbuthnott J. P., Gemmell C. G., Kent J., Lyell A. 1969; Haemolysin and enzyme patterns of coagulase-positive staphylococci isolated from toxic epidermal necrolysis, Ritter’s disease and impetigo contagiosa. j. med. Microbiol 2:479
     [Google Scholar]
  4. Baird-Parker A. C. 1963; A classification of micrococci and staphylococci based on physiological and biochemical tests. J. gen. Microbiol 30:409
     [Google Scholar]
  5. Barber M., Kuper S. W. A. 1951; Identification of Staphylococcus pyogenes by the phosphatase reaction. J. Path. Bact 63:65
     [Google Scholar]
  6. Bautz E., Freese E. 1960; On the mutagenic effect of alkylating agents. Proc. natn Acad. Sci. USA 46:1585
     [Google Scholar]
  7. Blair J. E., Williams R. E. O. 1961; Phage typing of staphylococci. Bull. Wld Hlth Org 24:771
     [Google Scholar]
  8. Burnet F. M., Keogh E. V., Lush D. 1937; The immunological reactions of the filterable viruses. Aust. J. exp. Biol. med. Sci 15:231
     [Google Scholar]
  9. Cadness-Graves B., Williams R., Harper G. J., Miles A. A. 1943; Slide-test for coagulase-positive staphylococci. Lancet 1:736
     [Google Scholar]
  10. Cawdery M., Foster W. D., Hawgood B. C., Taylor C. 1969; The role of coagulase in the defence of Staphylococcus aureus against phagocytosis. Br. J. exp. Path 50:408
     [Google Scholar]
  11. Elek S. D., Levy E. 1950; Distribution of haemolysins in pathogenic and nonpathogenic staphylococci. J. Path. Bact 62:541
     [Google Scholar]
  12. Foster W. D. 1971; The relationship between the pathogenicity of Staphylococcus aureus for the mouse and its pathogenicity for man and the guinea pig. Br. J. exp. Path 52:307
     [Google Scholar]
  13. Gershon A., Gladstone G. P. 1968; Immunofluorescent demonstration of staphylococcal leucocidin components F and S. Br. J. exp. Path 49:477
     [Google Scholar]
  14. Gladstone G. P., van Heyningen W. E. 1957; Staphylococcal leucocidins. Br. J. exp. Path 38:123
     [Google Scholar]
  15. Gladstone G. P., Mudd S., Hochstein H. D., Lenhart N. A. 1962; The assay of anti-staphylococcal leucocidal components (F and S) in human serum. Br. J. exp. Path 43:295
     [Google Scholar]
  16. Hayes W. 1968; The genetics of bacteria and their viruses. 2nd ed 0Oxford and Edinburgh: p 307
     [Google Scholar]
  17. Hill M. J. 1968; A staphylococcal aggressin. J. med. Microbiol 1:33
     [Google Scholar]
  18. Jessen O., Rosendal K., Bulow P., Faber V., Eriksen K. R. 1969; Changing staphylococci and staphylococcal infections: a ten year study of bacteria and cases of bacteremia. New Eng. J. Med 281:627
     [Google Scholar]
  19. Kapral F. A., Li I. W. 1960; Virulence and coagulases of Staphylococcus aureus. Proc. Soc. exp. Biol. Med. 104:151
     [Google Scholar]
  20. Lack C. H., Wailling D. G. 1954; A study of 435 strains of Staphylococcus pyogenes with reference to factors which may contribute to pathogenicity. J. Path. Bact 68:431
     [Google Scholar]
  21. Loveless A., Howarth S. 1959; Mutation of bacteria at high levels of survival by ethyl methane sulphonate. Nature, Lond 184:1780
     [Google Scholar]
  22. McClatchy J. K., Rosenblum E. D. 1966; Biological properties of α-toxin mutants of Staphylococcus aureus. J. Bact 92:575
     [Google Scholar]
  23. Meynell G. G., Meynell E. 1965; Theory and practice in experimental bacteriology. Cambridge p 26
     [Google Scholar]
  24. Miles A. A., Misra S. S., Irwin J. O. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 38:732
     [Google Scholar]
  25. Mudd S., Gladstone G. P., Lenhart N. A. 1965; The antigenicity in man of staphylococcal leucocidin toxoid, with notes on therapeutic immunization in chronic osteomyelitis. Br. J. exp. Path 46:455
     [Google Scholar]
  26. Noble W. C. 1966; Virulence and the biochemical characters of staphylococci. J. Path. Bact 91:181
     [Google Scholar]
  27. Otsuji N., Sekiguchi M., Iijima T., Takagi Y. 1959; Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. Nature, Lond 184:1079
     [Google Scholar]
  28. Sierra G. 1957; A simple method for the detection of lipolytic activity of microorganisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23:15
     [Google Scholar]
  29. Skegg J. L., Anderson S. G. 1969; International reference preparation of antistaphylococcal P-V leucocidin serum. Bull. Wld Hlth Org 40:593
     [Google Scholar]
  30. Smith R. F., Willett N. P. 1968; Rapid plate method for screening hyaluronidase and chondroitin sulfatase-producing microorganisms. Appl. Microbiol 16:1434
     [Google Scholar]
  31. Vijver J. C. M. van der, Kraayeveld C. A., Michel M. F. 1972; A solid medium for visual demonstration of coagulase production by Staphylococcus aureus. J. clin. Path 25:450
     [Google Scholar]
  32. Vijver J. C. M. van der, van Es-Boon M. M., Michel M. F. 1972; Lysogenic conversion in Staphylococcus aureus to leucocidin production. J. Virol 10:318
     [Google Scholar]
  33. Vijver J. C. M. van der, van Es-Boon M. M., Michel M. F. 1974; The enhancing effect of cell-wall aggressin on the virulence of Staphylococcus aureus in normal and irradiated mice and in man. Antonie van Leeuwenhoek 40:49
     [Google Scholar]
  34. Vijver J. C. M. van der, van Es-Boon M. M., Michel M. F. 1975; A study of virulence factors with induced mutants of Staphylococcus aureus. J. med. Microbiol 8:279
     [Google Scholar]
  35. Williams R. E. O. 1963; Healthy carriage of Staphylococcus aureus: its prevalence and importance. Bact. Rev 27:56
     [Google Scholar]
  36. Winkler K. C., de Waart J., Grootsen C., Zegers B. J. M., Tellier N. F., Vertregt C. D. 1965; Lysogenic conversion of staphylococci to loss of ß-toxin. J. gen. Microbiol 39:321
     [Google Scholar]
  37. Wiseman G. M. 1970; The beta- and delta-toxins of Staphylococcus aureus. In Microbial toxins vol III: edited by Montie T. C., Kadis S., Ajl S. J. New York and London: p 237
     [Google Scholar]
  38. Woodin A. M. 1959; Fractionation of a leucocidin from Staphylococcus aureus. Biochem. J 73:225
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-8-2-265
Loading
INDUCTION OF MUTATION IN STAPHYLOCOCCUS AUREUS BY ETHYLMETHANE SULPHONATE
J. Med. Microbiol. 8, 265 (1975); https://doi.org/10.1099/00222615-8-2-265
/content/journal/jmm/10.1099/00222615-8-2-265
/content/journal/jmm/10.1099/00222615-8-2-265
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error