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f Probiotic attributes, antioxidant, anti-inflammatory and neuromodulatory effects of CFR 3003: and evidence

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  • Authors: G. Divyashri1​ ,2​ ,  G. Krishna3​ ,  Muralidhara3​ , S. G. Prapulla2​
    • VIEW AFFILIATIONS
    • 1​ 1​ Academy of Scientific and Innovative Research (AcSIR), New Delhi, India 2​ 2​ Microbiology and Fermentation Technology Department, CSIR-Central Food Technological Research Institute (CFTRI), Karnataka, Mysore 570 020, India 3​ 3​ Biochemistry and Nutrition Department, CSIR-Central Food Technological Research Institute (CFTRI), Karnataka, Mysore 570 020, India
  • S. G. Prapulla [email protected]
  • First Published Online: 01 December 2015, Journal of Medical Microbiology 64: 1527-1540, doi: 10.1099/jmm.0.000184
  • Subject: Prevention and Therapy
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Probiotic attributes, antioxidant, anti-inflammatory and neuromodulatory effects of CFR 3003: and evidence, Page 1 of 1

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  • Accumulating evidence suggests that probiotic bacteria play a vital role in modulating various aspects integral to the health and well-being of humans. In the present study, probiotic attributes and the antioxidant, anti-inflammatory and neuromodulatory potential of CFR 3003 were investigated by employing suitable model systems. exhibited robust resistance to gastrointestinal stress conditions as it could withstand acid stress at pH 1.5, 2 and 3. The bacterium also survived at a bile salt concentration of 0.45 %, and better tolerance was observed towards pepsin and trypsin. produced lactic acid as a major metabolic product, followed by butyric acid. Lyophilized cell-free supernatant (LCS) of exhibited significant antioxidant capacity evaluated against 1,1-diphenyl-2-picryl-hydrazyl, ascorbate auto-oxidation, oxygen radical absorbance and reducing power. Interestingly, , GG MTCC 1408 and LCS showed a significant anti-inflammatory effect by negatively modulating TNF-α production and upregulating IL-10 levels in LPS-stimulated macrophage cell lines. In an mice model, the propensity of probiotic supplements to modulate endogenous oxidative markers and redox status in brain regions was assessed. Young mice provided with oral supplements (daily for 28 days) of and exhibited diminished oxidative markers in the brain and enhanced activities of antioxidant enzymes with a concomitant increase in γ-aminobutyric acid and dopamine levels. Collectively, our findings clearly suggest the propensity of these bacteria to protect against tissue damage mediated through free radicals and inflammatory cytokines. Although the underlying molecular mechanisms need further studies, it is tempting to speculate that probiotics confer a neuroprotective advantage against oxidative damage-mediated neurodegenerative conditions.

  • Abbreviations:
    AChE acetylcholine esterase
    CAT catalase
    DA dopamine
    DCF 2′,7′-dichlorofluorescein
    DPPH 1,1-diphenyl-2-picryl-hydrazyl
    GABA γ-aminobutyric acid
    GSH glutathione
    GST glutathione -transferase
    LCS lyophilized cell-free supernatant
    ORAC oxygen radical absorbance capacity
    ROS reactive oxygen species
    TCA trichloroacetic acid
    TE Trolox equivalent

© 2015 The Authors | Published by the Microbiology Society

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2019-07-23
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