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oa Evaluation of fast-acting bactericidal activity and substantivity of an antiseptic agent, olanexidine gluconate, using an ex vivo skin model
- Authors: Hisae Nishioka1,* , Akihiro Nagahama1 , Yasuhide Inoue1 , Akifumi Hagi1
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1 Naruto Research Institute, Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima, Japan
- *Correspondence: Hisae Nishioka [email protected]
- First Published Online: 07 November 2018, Journal of Medical Microbiology 67: 1796-1803, doi: 10.1099/jmm.0.000870
- Subject: Prevention and Therapy
- Received:
- Accepted:
- Cover date:
- This is an open access article published by the Microbiology Society under the Creative Commons Attribution License




Evaluation of fast-acting bactericidal activity and substantivity of an antiseptic agent, olanexidine gluconate, using an ex vivo skin model, Page 1 of 1
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Purpose. We assessed the fast-acting bactericidal activity and substantivity of olanexidine gluconate (OLG) to investigate its remaining bactericidal activity on the skin after rinsing and drying by using an ex vivo Yucatan micropig (YMP) skin model.
Methodology. The fast-acting bactericidal activity was evaluated in pigskin models inoculated with methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, vancomycin-resistant Enterococcus faecalis (VRE), Acinetobacter baumannii, Corynebacterium minutissimum and Cutibacterium acnes. To evaluate substantivity, the YMP skin piece first had 1.5 % OLG, chlorhexidine gluconate (CHG) formulations or 10 % povidone–iodine (PVP-I) applied to it, and was then rinsed with distilled water, incubated for 4, 6, 8 or 12 h and inoculated with the test bacteria (MRSA, S. epidermidis and VRE). The viable bacteria remaining at 1 min of exposure of bacteria were counted to measure the quantity of antiseptic molecules retaining bactericidal activity. To determine the factors contributing to the substantivity, the stratum corneum (SC) of the YMP skin that had had OLG or CHG applied to it was exfoliated using a tape-stripping method and the amount of antiseptic was quantitated.
Results. OLG showed a fast-acting bactericidal activity that was similar to or stronger than that of CHG formulations up to a concentration of 1 % and PVP-I with a short exposure time of 30 s, and substantivity until 12 h after rinsing, whereas the other antiseptics hardly showed any substantivity. There was 2.8 times or more OLG in the SC than CHG.
Conclusion. OLG has fast-acting activity and substantivity, which are required properties for an antiseptic, and is useful for preventing infections.
- Keyword(s): fast-acting effect, bactericidal effect, substantivity, olanexidine, ex vivo model, antiseptic
© 2018 The Authors | Published by the Microbiology Society
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