1887

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Volume 62, Issue 2

A mutant in the Fur-regulated virulence locus () induces cellular immunity and confers protection against listeriosis in mice Free

Abstract

is a Gram-positive intracellular pathogen that is responsible for listeriosis, a potentially fatal, food-borne illness. Due to its cytoplasmic location during infection, this pathogen can mediate a long-lasting cellular immune response, which makes attenuated strains strong candidates for vaccine development. Recently, our group identified and characterized (Fur-regulated virulence factor A), and deletion of this gene resulted in disruption of iron homeostasis and a strong attenuation in virulence. Despite significant attenuation in the mouse infection model, the mutant was capable of intracellular growth in antigen-presenting cells. Indeed, mice immunized with Δ were able to effectively stimulate specific CD8 T cells to the listerial epitopes LLO and P60 at levels comparable with strain EGDe. Most notably, mice immunized with Δ then subsequently challenged with the wild-type strain were completely protected from listerial infection. On the basis of these results, we advocate the use of Δ as a live attenuated listerial vaccine, and propose that this mutant may serve as a platform for the development of a future vaccine delivery vehicle.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-02-01
2024-05-01
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A mutant in the Listeria monocytogenes Fur-regulated virulence locus (frvA) induces cellular immunity and confers protection against listeriosis in mice
J. Med. Microbiol. 62, 185 (2013); https://doi.org/10.1099/jmm.0.049114-0
/content/journal/jmm/10.1099/jmm.0.049114-0
/content/journal/jmm/10.1099/jmm.0.049114-0
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