1887

Abstract

The ability of the RD (rhabdomyosarcoma) and MRC-5 cell-lines to detect enteroviruses in 33 clinical samples (cerebrospinal fluid, stools and throat swabs) was evaluated. The samples had previously tested enterovirus-positive by traditional tube-culture and had been frozen after their initial processing. By traditional tube-culture, 100 and 85 % of samples were positive for enterovirus in RD and MRC-5 cells, respectively. By rapid shell-vial assay, 94 and 45.5 % were positive after 48 h incubation in RD and MRC-5 cells, respectively. RD cells supported growth of all enterovirus serotypes, whereas MRC-5 cells were not able to detect any of the three coxsackieviruses that were found (one coxsackievirus A9 and two coxsackievirus B5). The shell-vial assay with RD cell-lines may be a useful tool for rapid diagnosis of enteroviral infection.

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2003-09-01
2024-05-02
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References

  1. Berlin L. E, Rorabaugh M. L, Heldrich F, Roberts K, Doran T, Modlin J. F. 1993; Aseptic meningitis in infants < 2 years of age: diagnosis and etiology. J Infect Dis 168:888–892 [CrossRef]
    [Google Scholar]
  2. Buck G. E, Wiesemann M, Stewart L. 2002; Comparison of mixed cell culture containing genetically engineered BGMK and CaCo-2 cells (Super E-Mix) with RT-PCR and conventional cell culture for the diagnosis of enterovirus meningitis. J Clin Virol 25:Suppl. 1S13–S18
    [Google Scholar]
  3. Chonmaitree T, Ford C, Sanders C, Lucia H. L. 1988; Comparison of cell cultures for rapid isolation of enteroviruses. J Clin Microbiol 26:2576–2580
    [Google Scholar]
  4. Clarke L. M, McPhee J. M. G, Cummings R. V. 1992; Isolation of viruses in conventional tube culture: selection and inoculation of cell cultures. In Clinical Microbiology Procedures Handbook pp. 8.5.1–8.5.13 Edited by Isenberg H. D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Dagan R, Menegus M. A. 1986; A combination of four cell types for rapid detection of enteroviruses in clinical specimens. J Med Virol 19:219–228 [CrossRef]
    [Google Scholar]
  6. Hsiung G. D. 1994; Picornaviridae . In Hsiung's Diagnostic Virology: as Illustrated by Light and Electron Microscopy . , 4th edn. pp 119–140 Edited by Fong C. K. Y., Landry M. L., Hsiung G. D. New Haven: Yale University Press;
  7. Huang Y. T, Yam P, Yan H, Sun Y. 2002; Engineered BGMK cells for sensitive and rapid detection of enteroviruses. J Clin Microbiol 40:366–371 [CrossRef]
    [Google Scholar]
  8. King A. M. Q, Brown F, Christian P. G. 8 other authors 2000; Picornaviridae . In Virus Taxonomy. Seventh Report of the International Committee for the Taxonomy of Viruses pp 657–673 Edited by Regenmortel M. H. V. Van. others New York: Academic Press;
    [Google Scholar]
  9. Klespies S. L, Cebula D. E, Kelley C. L, Galehouse D, Maurer C. C. 1996; Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay. J Clin Microbiol 34:1465–1467
    [Google Scholar]
  10. Lipson S. M, David K, Shaikh F, Qian L. 2001; Detection of precytopathic effect of enteroviruses in clinical specimens by centrifugation-enhanced antigen detection. J Clin Microbiol 39:2755–2759 [CrossRef]
    [Google Scholar]
  11. Marshall G. S, Hauck M. A, Buck G, Rabalais G. P. 1997; Potential cost savings through rapid diagnosis of enteroviral meningitis. Pediatr Infect Dis J 16:1086–1087 [CrossRef]
    [Google Scholar]
  12. Melnick J. L. 1996; Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Fields Virology, 3rd edn. pp 655–712 Edited by Fields B. N., Knipe D. M., Howley P. M., Chanock R. M., Monath T. P., Melnick J. L., Roizman B., Straus S. E. Philadelphia: Lippincott-Raven;
    [Google Scholar]
  13. Reina J, Ballesteros F, Munar M, Mari M, Subirats M. 2000; Evaluation of different clinical samples and cell lines in the isolation of enterovirus in pediatric patients. Enferm Infecc Microbiol Clin 18:116–119 in Spanish
    [Google Scholar]
  14. Romero J. R, Rotbart H. A. 2003; Enteroviruses. In Manual of Clinical Microbiology , 8th edn. pp 1427–1438 Edited by Murray P. M., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Rotbart H. A. 1995; Enteroviral infections of the central nervous system. Clin Infect Dis 20:971–981 [CrossRef]
    [Google Scholar]
  16. Sawyer M. H. 1999; Enterovirus infections: diagnosis and treatment. Pediatr Infect Dis J 18:1033–1040 [CrossRef]
    [Google Scholar]
  17. Terletskaia-Ladwig E, Metzger C, Schalasta G, Enders G. 2000; A new enzyme immunoassay for the detection of enteroviruses in faecal specimens. J Med Virol 60:439–445 [CrossRef]
    [Google Scholar]
  18. Van Doornum G. J. J, De Jong J. C. 1998; Rapid shell vial culture technique for detection of enteroviruses and adenoviruses in fecal specimens: comparison with conventional virus isolation method. J Clin Microbiol 36:2865–2868
    [Google Scholar]
  19. Verstrepen W. A, Bruynseels P, Mertens A. H. 2002; Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens. J Clin Virol 25:Suppl. 1S39–S43
    [Google Scholar]
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