1887

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Volume 63, Issue 8

Probiotic assessment of 6HL and 2HL isolated from vaginal microflora Free

Abstract

Forty-five lactic acid bacteria (LAB) were isolated from the vaginal specimens of healthy fertile women, and the identities of the bacteria were confirmed by sequencing of their 16S rDNA genes. Among these bacteria, only four isolates were able to resist and survive in low pH, bile salts and simulated digestion conditions. 2HL, 6HL, 36YL and 5BL showed the best resistance to these conditions. These strains were evaluated further to assess their ability to adhere to human intestinal Caco-2 cells. 2HL and 6HL were the most adherent strains. tests under neutralized pH proved the antimicrobial activity of both strains. Results revealed that the growth of O26, and was suppressed by both LAB strains. The antibiotic susceptibility tests showed that these strains were sensitive to all nine antibiotics: vancomycin, tetracycline, ampicillin, penicillin, gentamicin, erythromycin, clindamycin, sulfamethoxazole and chloramphenicol. These data suggest that 6HL and 2HL could be examined further for their useful properties and could be developed as new probiotics.

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© 2014 The Authors
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2014-08-01
2024-04-28
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Probiotic assessment of Enterococcus durans 6HL and Lactococcus lactis 2HL isolated from vaginal microflora
J. Med. Microbiol. 63, 1044 (2014); https://doi.org/10.1099/jmm.0.074161-0
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